{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:37:22Z","timestamp":1760099842246},"reference-count":36,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,4,15]]},"abstract":"<jats:p>We report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs of short disintegrins and those coding for precursors of dimeric disintegrin chains. The Macrovipera lebetina transmediterranea dimeric disintegrin reported to inhibit the laminin-binding integrins \u03b13\u03b21, \u03b16\u03b21 and \u03b17\u03b21 was analysed using a proteomic approach and was shown to bear MLD (Met-Leu-Asp) and VGD (Val-Gly-Asp) motifs. The results highlight the fact that disintegrins have evolved a restricted panel of integrin-blocking sequences that segregate with defined branches of the phylogenetic tree of the integrin \u03b1-chains, providing novel insights into the evolutionary adaptation of the snake venom antagonists to the ligand-binding sites of their target integrin receptors.<\/jats:p>","DOI":"10.1042\/bj20051678","type":"journal-article","created":{"date-parts":[[2006,3,28]],"date-time":"2006-03-28T12:39:20Z","timestamp":1143549560000},"page":"385-392","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Molecular cloning of disintegrins from <i>Cerastes vipera<\/i> and <i>Macrovipera lebetina transmediterranea<\/i> venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system"],"prefix":"10.1042","volume":"395","author":[{"given":"Libia","family":"Sanz","sequence":"first","affiliation":[{"name":"Instituto de Biomedicina de Valencia, C.S.I.C., Jaume Roig 11, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amine","family":"Bazaa","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naziha","family":"Marrakchi","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Valencia, C.S.I.C., Jaume Roig 11, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Chenik","sequence":"additional","affiliation":[{"name":"Laboratoire d'immunologie, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zakaria","family":"Bel\u00a0Lasfer","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"El\u00a0Ayeb","sequence":"additional","affiliation":[{"name":"Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, B.P. 74, 1002 Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan\u00a0J.","family":"Calvete","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Valencia, C.S.I.C., Jaume Roig 11, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2006,3,28]]},"reference":[{"key":"2021112214103165800_B1","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0041-0101(98)00126-3","article-title":"Snake venoms and the hemostatic system","volume":"36","author":"Markland","year":"1998","journal-title":"Toxicon"},{"key":"2021112214103165800_B2","article-title":"Perspectives in Molecular Toxinology","author":"M\u00e9nez","year":"2002"},{"key":"2021112214103165800_B3","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/pmic.200300628","article-title":"Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and tandem mass spectrometry analysis","volume":"4","author":"Ju\u00e1rez","year":"2004","journal-title":"Proteomics"},{"key":"2021112214103165800_B4","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/j.toxicon.2005.02.030","article-title":"Snake venom and coagulopathy","volume":"45","author":"White","year":"2005","journal-title":"Toxicon"},{"key":"2021112214103165800_B5","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/BF02338834","article-title":"Evolution of disintegrin cysteine-rich and mammalian matrix-degrading metalloproteinases: gene duplication and divergence of a common ancestor rather than convergent evolution","volume":"43","author":"Moura-da-Silva","year":"1996","journal-title":"J. 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