{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:06:17Z","timestamp":1782399977011,"version":"3.54.5"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,5,15]]},"abstract":"<jats:p>Cyclophilins constitute a family of proteins involved in many essential cellular functions. They have also been identified as a panallergen family able to elicit IgE-mediated hypersensitivity reactions. Moreover, it has been shown that human cyclophilins are recognized by serum IgE from patients sensitized to environmental cyclophilins. IgE-mediated autoreactivity to self-antigens that have similarity to environmental allergens is often observed in atopic disorders. Therefore comparison of the crystal structure of human proteins with similarity to allergens should allow the identification of structural similarities to rationally explain autoreactivity. A new cyclophilin from Aspergillus fumigatus (Asp f 27) has been cloned, expressed and showed to exhibit cross-reactivity in vitro and in vivo. The three-dimensional structure of cyclophilin from the yeast Malassezia sympodialis (Mala s 6) has been determined at 1.5\u00a0\u00c5 (1\u00a0\u00c5=0.1\u00a0nm) by X-ray diffraction. Crystals belong to space group P41212 with unit cell dimensions of a=b=71.99\u00a0\u00c5 and c=106.18\u00a0\u00c5. The structure was solved by molecular replacement using the structure of human cyclophilin A as the search model. The refined structure includes all 162 amino acids of Mala s 6, an active-site-bound Ala-Pro dipeptide and 173 water molecules, with a crystallographic R- and free R-factor of 14.3% and 14.9% respectively. The overall structure consists of an eight-stranded antiparallel \u03b2-barrel and two \u03b1-helices covering the top and bottom of the barrel, typical for cyclophilins. We identified conserved solvent-exposed residues in the fungal and human structures that are potentially involved in the IgE-mediated cross-reactivity.<\/jats:p>","DOI":"10.1042\/bj20051708","type":"journal-article","created":{"date-parts":[[2006,4,28]],"date-time":"2006-04-28T18:24:37Z","timestamp":1146248677000},"page":"41-49","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Analysis of the cross-reactivity and of the 1.5\u00a0\u00c5 crystal structure of the <i>Malassezia sympodialis<\/i> Mala s 6 allergen, a member of the cyclophilin pan-allergen family"],"prefix":"10.1042","volume":"396","author":[{"given":"Andreas\u00a0G.","family":"Glaser","sequence":"first","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Limacher","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland"},{"name":"Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH), CH-8093 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Fl\u00fcckiger","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Scheynius","sequence":"additional","affiliation":[{"name":"Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet, S-171 76 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Scapozza","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Th\u00e9rapeutique, Section des Sciences Pharmaceutiques, Universit\u00e9 de Gen\u00e8ve, \u00c9cole de Pharmacie Gen\u00e8ve Lausanne, Quai Ernest-Ansermet 30, CH-1211 Gen\u00e8ve 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reto","family":"Crameri","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF), CH-7270 Davos, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2006,4,26]]},"reference":[{"key":"2021112213065121100_B1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/337473a0","article-title":"Peptidyl-prolyl cis\u2013trans isomerase is the cyclosporin A-binding protein cyclophilin","volume":"337","author":"Takahashi","year":"1989","journal-title":"Nature (London)"},{"key":"2021112213065121100_B2","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1096\/fasebj.6.15.1464374","article-title":"Structural and evolutionary relationships among the immunophilins: two ubiquitous families of peptidyl-prolyl cis\u2013trans isomerases","volume":"6","author":"Trandinh","year":"1992","journal-title":"FASEB J."},{"key":"2021112213065121100_B3","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/0092-8674(91)90124-H","article-title":"Calcineurin is a common target of cyclophilin\u2013cyclosporin A and KFBP\u2013FK506 complexes","volume":"66","author":"Liu","year":"1991","journal-title":"Cell"},{"key":"2021112213065121100_B4","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/365352a0","article-title":"The T-cell transcription factor NAFTp is a substrate for calcineurin and interacts with Fos and Jun","volume":"365","author":"Jain","year":"1993","journal-title":"Nature (London)"},{"key":"2021112213065121100_B5","first-page":"259","article-title":"Pharmacology of cyclosporine (sandimmune). 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