{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:19:59Z","timestamp":1762078799396},"reference-count":53,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,6,15]]},"abstract":"<jats:p>Apyrase activity is present in the saliva of haematophagous arthropods. It is related to blood-feeding because of the apyrase ability to hydrolyse ADP, a key component of platelet aggregation. Five apyrases with apparent molecular masses of 88, 82, 79, 68 and 67\u00a0kDa were identified in the saliva of the vector of Chagas disease, Triatoma infestans. The large size observed during purification of these enzymes suggested oligomerization. In the present study, we confirmed, using gel-filtration and analytical ultracentrifugation, the presence of apyrase oligomers with molecular masses of 200\u00a0kDa in the saliva. Electrophoretic analyses showed that disulphide bonds were involved in homo-oligomerization. In addition, heterogeneity in disulphide bonds and in pI was detected, with the pI ranging from 4.9 to 5.4. The present study gives the first insights into the quaternary structure of soluble apyrases.<\/jats:p>","DOI":"10.1042\/bj20052019","type":"journal-article","created":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T12:04:58Z","timestamp":1148990698000},"page":"509-515","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Salivary apyrases of <i>Triatoma infestans<\/i> are assembled into homo-oligomers"],"prefix":"10.1042","volume":"396","author":[{"given":"Eric","family":"Faudry","sequence":"first","affiliation":[{"name":"Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, Brazil 70.910-900"},{"name":"Laboratoire d'Ing\u00e9nierie des Macromol\u00e9cules, Institut de Biologie Structurale J.P. Ebel UMR5075 CEA\/CNRS\/UJF, 38027 Grenoble cedex 1, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime\u00a0M.","family":"Santana","sequence":"additional","affiliation":[{"name":"Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, Brazil 70.910-900"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Ebel","sequence":"additional","affiliation":[{"name":"Laboratoire de Biophysique Mol\u00e9culaire, Institut de Biologie Structurale J.P. Ebel UMR5075 CEA\/CNRS\/UJF, 38027 Grenoble cedex 1, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Vernet","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ing\u00e9nierie des Macromol\u00e9cules, Institut de Biologie Structurale J.P. 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