{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:39:01Z","timestamp":1791272341746,"version":"4.1.0"},"reference-count":55,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,9,15]]},"abstract":"<jats:p>S1P (sphingosine 1-phosphate) receptor expression and the effects of S1P on migration were studied in one papillary (NPA), two follicular (ML-1, WRO) and two anaplastic (FRO, ARO) thyroid cancer cell lines, as well as in human thyroid cells in primary culture. Additionally, the effects of S1P on proliferation, adhesion and calcium signalling were addressed in ML-1 and FRO cells. All cell types expressed multiple S1P receptors. S1P evoked intracellular calcium signalling in primary cultures, ML-1 cells and FRO cells. Neither proliferation nor migration was affected in primary cultures, whereas S1P partly inhibited proliferation in ML-1 and FRO cells. Low nanomolar concentrations of S1P inhibited migration in FRO, WRO and ARO cells, but stimulated ML-1 cell migration. Consistently, S1P1 and S1P3, which mediate migratory responses, were strongly expressed in ML-1 cells, and S1P2, which inhibits migration, was the dominating receptor in the other cell lines. The migratory effect in ML-1 cells was mediated by Gi and phosphatidylinositol 3-kinase. Both S1P and the S1P1-specific agonist SEW-2871 induced Akt phosphorylation at Ser473. However, SEW-2871 failed to stimulate migration, whereas the S1P1\/S1P3 antagonist VPC 23019 inhibited S1P-induced migration. The results suggest that aberrant S1P receptor expression may enhance thyroid cancer cell migration and thus contribute to the metastatic behaviour of some thyroid tumours.<\/jats:p>","DOI":"10.1042\/bj20060299","type":"journal-article","created":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T10:45:57Z","timestamp":1157021157000},"page":"547-556","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Sphingosine 1-phosphate receptor expression profile and regulation of migration in human thyroid cancer cells"],"prefix":"10.1042","volume":"398","author":[{"given":"Sonja","family":"Balthasar","sequence":"first","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Samulin","sequence":"additional","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Ahlgren","sequence":"additional","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Bergelin","sequence":"additional","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Lundqvist","sequence":"additional","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil\u00a0C.","family":"Toescu","sequence":"additional","affiliation":[{"name":"Division of Medical Sciences, University of Birmingham, Birmingham B15 2TT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret\u00a0C.","family":"Eggo","sequence":"additional","affiliation":[{"name":"Division of Medical Sciences, University of Birmingham, Birmingham B15 2TT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kid","family":"T\u00f6rnquist","sequence":"additional","affiliation":[{"name":"Department of Biology, \u00c5bo Akademi University, 20520 Turku, Finland"},{"name":"Minerva Foundation Institute for Medical Research, Biomedicum Helsinki, 00270 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2006,8,29]]},"reference":[{"key":"2021112213390093400_B1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1002\/jcb.20094","article-title":"G protein mediated signaling pathways in lysophospholipid induced cell proliferation and survival","volume":"92","author":"Radeff-Huang","year":"2004","journal-title":"J. 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