{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:25:37Z","timestamp":1770218737075,"version":"3.49.0"},"reference-count":51,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,3,1]]},"abstract":"<jats:p>We have demonstrated recently that CICR (Ca2+-induced Ca2+ release) activity of RyR1 (ryanodine receptor 1) is held to a low level in mammalian skeletal muscle (\u2018suppression\u2019 of the channel) and that this is largely caused by the interdomain interaction within RyR1 [Murayama, Oba, Kobayashi, Ikemoto and Ogawa (2005) Am. J. Physiol. Cell Physiol. 288, C1222\u2013C1230]. To test the hypothesis that aberration of this suppression mechanism is involved in the development of channel dysfunctions in MH (malignant hyperthermia), we investigated properties of the RyR1 channels from normal and MHS (MH-susceptible) pig skeletal muscles with an Arg615\u2192Cys mutation using [3H]ryanodine binding, single-channel recordings and SR (sarcoplasmic reticulum) Ca2+ release. The RyR1 channels from MHS muscle (RyR1MHS) showed enhanced CICR activity compared with those from the normal muscle (RyR1N), although there was little or no difference in the sensitivity to several ligands tested (Ca2+, Mg2+ and adenine nucleotide), nor in the FKBP12 (FK506-binding protein 12) regulation. DP4, a domain peptide matching the Leu2442\u2013Pro2477 region of RyR1 which was reported to activate the Ca2+ channel by weakening the interdomain interaction, activated the RyR1N channel in a concentration-dependent manner, and the highest activity of the affected channel reached a level comparable with that of the RyR1MHS channel with no added peptide. The addition of DP4 to the RyR1MHS channel produced virtually no further effect on the channel activity. These results suggest that stimulation of the RyR1MHS channel caused by affected inter-domain interaction between regions 1 and 2 is an underlying mechanism for dysfunction of Ca2+ homoeostasis seen in the MH phenotype.<\/jats:p>","DOI":"10.1042\/bj20061040","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T13:56:42Z","timestamp":1171547802000},"page":"349-357","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Postulated role of interdomain interaction between regions 1 and 2 within type 1 ryanodine receptor in the pathogenesis of porcine malignant hyperthermia"],"prefix":"10.1042","volume":"402","author":[{"given":"Takashi","family":"Murayama","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Juntendo University School of Medicine, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiharu","family":"Oba","sequence":"additional","affiliation":[{"name":"Department of Regulatory Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Hara","sequence":"additional","affiliation":[{"name":"Miyazaki Station National Livestock Breeding Center, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kikuo","family":"Wakebe","sequence":"additional","affiliation":[{"name":"Miyazaki Station National Livestock Breeding Center, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Ikemoto","sequence":"additional","affiliation":[{"name":"Boston Biomedical Research Institute, Watertown, MA 02472, U.S.A."},{"name":"Harvard Medical School, Boston, MA 02115, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Juntendo University School of Medicine, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2007,2,12]]},"reference":[{"key":"2021112213165102100_B1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1146\/annurev.ph.56.030194.002413","article-title":"Ryanodine receptor\/Ca2+ release channels and their regulation by endogenous effectors","volume":"56","author":"Meissner","year":"1994","journal-title":"Annu. 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