{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:37:01Z","timestamp":1753871821419,"version":"3.41.2"},"reference-count":13,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,11,15]]},"abstract":"<jats:p>Dietary and biliary cholesterol are taken up by intestinal epithelial cells and transported to the endoplasmic reticulum. At the endoplasmic reticulum, cholesterol is esterified, packaged into chylomicrons and secreted into the lymph for delivery to the bloodstream. NPC1L1 (Niemann\u2013Pick C1-like 1) is a protein on the enterocyte brush-border membrane that facilitates cholesterol absorption. Cholesterol's itinerary as it moves to the endoplasmic reticulum is unknown, as is the identity of any cellular proteins that facilitate the movement. Two proteins that play an important role in intracellular cholesterol transport and could potentially influence NPC1L1-mediated cholesterol uptake are NPC1 and NPC2 (Niemann\u2013Pick type C disease proteins 1 and 2). In this issue of the Biochemical Journal, Dixit and colleagues show that the absence or presence of NPC1 and NPC2 has no effect on intestinal cholesterol absorption in the mouse. Thus neither protein fills the gap in our knowledge of intra-enterocyte cholesterol transport. Furthermore, the NPC1\/NPC2 pathway would not be a good target for limiting the uptake of dietary cholesterol.<\/jats:p>","DOI":"10.1042\/bj20071340","type":"journal-article","created":{"date-parts":[[2007,10,29]],"date-time":"2007-10-29T14:59:59Z","timestamp":1193669999000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A role for NPC1 and NPC2 in intestinal cholesterol absorption \u2013 the hypothesis gutted"],"prefix":"10.1042","volume":"408","author":[{"given":"Laura","family":"Liscum","sequence":"first","affiliation":[{"name":"Department of Physiology, Tufts University School of Medicine, Boston, MA 02111, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2007,10,29]]},"reference":[{"key":"2021112213352864800_B1","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1126\/science.277.5323.228","article-title":"Niemann\u2013Pick C1 disease gene: homology to mediators of cholesterol homeostasis","volume":"277","author":"Carstea","year":"1997","journal-title":"Science"},{"key":"2021112213352864800_B2","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1126\/science.290.5500.2298","article-title":"Identification of HE1 as the second gene of Niemann\u2013Pick C disease","volume":"290","author":"Naureckiene","year":"2000","journal-title":"Science"},{"key":"2021112213352864800_B3","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1126\/science.1093131","article-title":"Niemann-Pick C1-like 1 protein is critical for intestinal cholesterol absorption","volume":"303","author":"Altmann","year":"2004","journal-title":"Science"},{"key":"2021112213352864800_B4","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1006\/geno.2000.6151","article-title":"Evidence for a Niemann\u2013Pick C (NPC) gene family: identification and characterization of NPC1L1","volume":"65","author":"Davies","year":"2000","journal-title":"Genomics"},{"key":"2021112213352864800_B5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/BJ20071167","article-title":"Do mammalian NPC1 and NPC2 play a role in intestinal cholesterol absorption?","volume":"408","author":"Dixit","year":"2007","journal-title":"Biochem. 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