{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:06:09Z","timestamp":1791270369726,"version":"4.1.0"},"reference-count":41,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,2,15]]},"abstract":"<jats:p>AXHs (arabinoxylan arabinofuranohydrolases) are \u03b1-L-arabinofuranosidases that specifically hydrolyse the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis was recently shown to produce an AXH that cleaves arabinose units from O-2- or O-3-mono-substituted xylose residues: BsAXH-m2,3 (B. subtilis AXH-m2,3). Crystallographic analysis reveals a two-domain structure for this enzyme: a catalytic domain displaying a five-bladed \u03b2-propeller fold characteristic of GH (glycoside hydrolase) family 43 and a CBM (carbohydrate-binding module) with a \u03b2-sandwich fold belonging to CBM family 6. Binding of substrate to BsAXH-m2,3 is largely based on hydrophobic stacking interactions, which probably allow the positional flexibility needed to hydrolyse both arabinose substituents at the O-2 or O-3 position of the xylose unit. Superposition of the BsAXH-m2,3 structure with known structures of the GH family 43 exo-acting enzymes, \u03b2-xylosidase and \u03b1-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone.<\/jats:p>","DOI":"10.1042\/bj20081256","type":"journal-article","created":{"date-parts":[[2008,11,5]],"date-time":"2008-11-05T18:20:32Z","timestamp":1225909232000},"page":"39-47","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["Structural analysis of a glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase in complex with xylotetraose reveals a different binding mechanism compared with other members of the same family"],"prefix":"10.1042","volume":"418","author":[{"given":"Elien","family":"Vandermarliere","sequence":"first","affiliation":[{"name":"Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O&N II, bus 822, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tine\u00a0M.","family":"Bourgois","sequence":"additional","affiliation":[{"name":"Laboratory of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, bus 2462, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martyn\u00a0D.","family":"Winn","sequence":"additional","affiliation":[{"name":"Computational Science and Engineering Department, STFC Daresbury Laboratory, Daresbury, Warrington WA4 4AD, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"van\u00a0Campenhout","sequence":"additional","affiliation":[{"name":"Laboratory of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, bus 2462, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Volckaert","sequence":"additional","affiliation":[{"name":"Laboratory of Gene Technology, Department of Biosystems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, bus 2462, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan\u00a0A.","family":"Delcour","sequence":"additional","affiliation":[{"name":"Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, bus 2463, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei\u00a0V.","family":"Strelkov","sequence":"additional","affiliation":[{"name":"Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O&N II, bus 822, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Rabijns","sequence":"additional","affiliation":[{"name":"Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Herestraat 49, O&N II, bus 822, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe\u00a0M.","family":"Courtin","sequence":"additional","affiliation":[{"name":"Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, bus 2463, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2009,1,28]]},"reference":[{"key":"2021112214151950100_B1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cbpa.2006.02.035","article-title":"Bioethanol","volume":"10","author":"Gray","year":"2006","journal-title":"Curr. 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