{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:04:19Z","timestamp":1792202659059,"version":"4.4.0"},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,4,1]]},"abstract":"<jats:p>Prx (peroxiredoxin) is a multifunctional redox protein with thioredoxin-dependent peroxidase activity. Prx4 is present as a secretory protein in most tissues, whereas in sexually mature testes it is anchored in the ER (endoplasmic reticulum) membrane of spermatogenic cells via an uncleaved N-terminal hydrophobic peptide. We generated a Prx4 knockout mouse to investigate the function of Prx4 in vivo. Prx4\u2212\/y mice lacking Prx4 expression in all cells were obtained by mating Prx4flox\/+ female mice with Cre-transgenic male mice that ubiquitously expressed Cre recombinase. The resulting Prx4\u2212\/y male mice were fertile, and most organs were nearly normal in size, except for testicular atrophy. The number of deoxynucleotidyl transferase-mediated dUTP nick end labelling-positive spermatogenic cells was higher in Prx4\u2212\/y mice than in Prx4+\/y mice and increased remarkably in response to warming the lower abdomen at 43\u00a0\u00b0C for 15\u00a0min. Cells reactive to antibodies against 4-hydroxynonenal and 8-hydroxyguanine were high in the Prx4\u2212\/y mice and concomitant with elevated oxidation of lipid and protein thiols. The cauda epididymis of Prx4\u2212\/y mice contained round spermatocytes, which were not found in Prx4+\/y mice, and displayed oligozoospermia. However, mature spermatozoa from the epididymis of Prx4\u2212\/y mice exhibited normal fertilization In vitro. Taken together, these results indicate that spermatogenic cells lacking Prx4 are more susceptible to cell death via oxidative damage than their wild-type counterparts. Our results suggest that the presence of Prx4, most likely the membrane-bound form, is important for spermatogenesis, but not an absolute requisite.<\/jats:p>","DOI":"10.1042\/bj20081526","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T10:22:09Z","timestamp":1230114129000},"page":"149-158","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":177,"title":["<i>Peroxiredoxin 4<\/i>knockout results in elevated spermatogenic cell death via oxidative stress"],"prefix":"10.1042","volume":"419","author":[{"given":"Yoshihito","family":"Iuchi","sequence":"first","affiliation":[{"name":"Department of Biomolecular Function, Graduate School of Medical Science, Yamagata University, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Futoshi","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Function, Graduate School of Medical Science, Yamagata University, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Tsunoda","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Function, Graduate School of Medical Science, Yamagata University, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Kibe","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Function, Graduate School of Medical Science, Yamagata University, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Shirasawa","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Structural Science, Yamagata University School of Medicine, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Okabe","sequence":"additional","affiliation":[{"name":"Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Function, Graduate School of Medical Science, Yamagata University, Yamagata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2009,3,13]]},"reference":[{"key":"2021112214214064400_B1","first-page":"246","article-title":"Free Radical Biology and Medicine","author":"Halliwell","year":"1999","edition":"3rd edn."},{"key":"2021112214214064400_B2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.ceb.2005.02.004","article-title":"Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins","volume":"17","author":"Rhee","year":"2005","journal-title":"Curr. 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