{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:42:00Z","timestamp":1791200520451,"version":"4.2.0"},"reference-count":44,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:p>Oxidative events involving band 3 (Anion Exchanger 1) have been associated with RBC (red blood cell) removal through binding of NAbs (naturally occurring antibodies); however, the underlying mechanism has been only partially characterized. In addition to inducing direct membrane protein oxidative modification, oxidative treatment specifically triggers the phosphorylation of band 3 tyrosine residues. The present study reports that diamide, a thiol group oxidant, induces disulfide cross-linking of poorly glycosylated band 3 and that the oligomerized band 3 fraction is selectively tyrosine phosphorylated both in G6PD (glucose-6-phosphate dehydrogenase)-deficient and control RBCs. This phenomenon is irreversible in G6PD-deficient RBCs, whereas it is temporarily limited in control RBCs. Diamide treatment caused p72 Syk phosphorylation and translocation to the membrane. Diamide also induced p72 Syk co-immunoprecipitation with aggregated band 3. Moreover, following size-exclusion separation of Triton X-100-extracted membrane proteins, Syk was found only in the high-molecular-mass fraction containing oligomerized\/phosphorylated band 3. Src family inhibitors efficiently abrogated band 3 tyrosine phosphorylation, band 3 clustering and NAbs binding to the RBC surface, suggesting a causal relationship between these events. Experiments performed with the non-permeant cross-linker BS3 (bis-sulfosuccinimidyl-suberate) showed that band 3 tyrosine phosphorylation enhances its capability to form large aggregates. The results of the present study suggest that selective tyrosine phosphorylation of oxidized band 3 by Syk may play a role in the recruitment of oxidized band 3 in large membrane aggregates that show a high affinity to NAbs, leading to RBC removal from the circulation.<\/jats:p>","DOI":"10.1042\/bj20081557","type":"journal-article","created":{"date-parts":[[2008,10,23]],"date-time":"2008-10-23T11:11:53Z","timestamp":1224760313000},"page":"359-367","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Oxidized and poorly glycosylated band 3 is selectively phosphorylated by Syk kinase to form large membrane clusters in normal and G6PD-deficient red blood cells"],"prefix":"10.1042","volume":"418","author":[{"given":"Antonella","family":"Pantaleo","sequence":"first","affiliation":[{"name":"Department of Genetics, Biology and Biochemistry, University of Turin, via Santena 5 bis, 10126 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Ferru","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, 37100 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Giribaldi","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology and Biochemistry, University of Turin, via Santena 5 bis, 10126 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franca","family":"Mannu","sequence":"additional","affiliation":[{"name":"Nurex s.r.l. 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Carta","sequence":"additional","affiliation":[{"name":"Nurex s.r.l. 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Matte","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, 37100 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"de\u00a0Franceschi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, 37100 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Turrini","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology and Biochemistry, University of Turin, via Santena 5 bis, 10126 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2009,2,11]]},"reference":[{"key":"2021112214242965400_B1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1179\/135100003225002998","article-title":"Haemoglobinopathies and resistance to malaria","volume":"8","author":"Roberts","year":"2003","journal-title":"Redox Rep."},{"key":"2021112214242965400_B2","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0140-6736(08)60073-2","article-title":"Glucose-6-phosphate dehydrogenase deficiency","volume":"371","author":"Cappellini","year":"2008","journal-title":"Lancet"},{"key":"2021112214242965400_B3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0014-5793(98)00185-9","article-title":"Plasmodium falciparum glutathione metabolism and growth are independent of glutathione system of host erythrocyte","volume":"424","author":"Ayi","year":"1998","journal-title":"FEBS Lett."},{"key":"2021112214242965400_B4","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.autrev.2008.03.017","article-title":"Naturally occurring anti-band 3 antibodies and red blood cell removal under physiological and pathological conditions","volume":"7","author":"Pantaleo","year":"2008","journal-title":"Autoimmun. 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