{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:55:06Z","timestamp":1791053706972,"version":"4.1.0"},"reference-count":52,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,3,15]]},"abstract":"<jats:p>In Ewing's sarcomas, chromosomal translocations cause the N-terminal domain of the EWS (Ewing's sarcoma protein) to fuse with the DNA-binding domains of the Ets (E26 transformation-specific) family of transcription factors. Here we show that EWS and EWS-Fli1 (Friend leukaemia virus integration 1), the fusion most frequently found in Ewing's sarcomas, become phosphorylated at Thr79 in response to either mitogens or DNA-damaging agents. The much weaker mitogen-induced phosphorylation of EWS is catalysed by the MAPKs (mitogen-activated protein kinases) ERK1 (extracellular signal-regulated kinase 1) and ERK2, whereas the much stronger phosphorylation of EWS induced by the DNA alkylating agent MMS (methyl methanesulphonate) can be catalysed by JNK (c-Jun N-terminal kinase) and at least one other protein kinase distinct from ERK1\/ERK2. In contrast, the phosphorylation of EWS-Fli1 induced by MMS was largely mediated by p38\u03b1\/p38\u03b2 MAPKs. MMS induced a much stronger phosphorylation of EWS-Fli1 than EWS in heterodimers comprising both proteins.<\/jats:p>","DOI":"10.1042\/bj20082097","type":"journal-article","created":{"date-parts":[[2008,12,16]],"date-time":"2008-12-16T14:29:43Z","timestamp":1229437783000},"page":"625-634","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Phosphorylation of Ewing's sarcoma protein (EWS) and EWS-Fli1 in response to DNA damage"],"prefix":"10.1042","volume":"418","author":[{"given":"Iva\u00a0V.","family":"Klevernic","sequence":"first","affiliation":[{"name":"MRC Protein Phosphorylation Unit, The Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Morton","sequence":"additional","affiliation":[{"name":"MRC Protein Phosphorylation Unit, The Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger\u00a0J.","family":"Davis","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Cohen","sequence":"additional","affiliation":[{"name":"MRC Protein Phosphorylation Unit, The Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2009,2,25]]},"reference":[{"key":"2021112214195781600_B1","first-page":"3087","article-title":"The EWS gene, involved in Ewing family of tumors, malignant melanoma of soft parts and desmoplastic small round cell tumors, codes for an RNA binding protein with novel regulatory domains","volume":"9","author":"Ohno","year":"1994","journal-title":"Oncogene."},{"key":"2021112214195781600_B2","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0888-7543(05)80363-5","article-title":"Genomic structure of the EWS gene and its relationship to EWSR1, a site of tumor-associated chromosome translocation","volume":"18","author":"Plougastel","year":"1993","journal-title":"Genomics"},{"key":"2021112214195781600_B3","first-page":"2690","article-title":"The Ewing's sarcoma gene product functions as a transcriptional activator","volume":"61","author":"Rossow","year":"2001","journal-title":"Cancer Res."},{"key":"2021112214195781600_B4","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1172\/JCI31222","article-title":"Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development","volume":"117","author":"Li","year":"2007","journal-title":"J. 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