{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:10:26Z","timestamp":1790651426832,"version":"4.1.0"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>The mechanisms of compartmentalization of intermediates and secretion of penicillins and cephalosporins in \u03b2-lactam antibiotic-producing fungi are of great interest. In Acremonium chrysogenum, there is a compartmentalization of the central steps of the CPC (cephalosporin C) biosynthetic pathway. In the present study, we found in the \u2018early\u2019 CPC cluster a new gene named cefP encoding a putative transmembrane protein containing 11 transmembrane spanner. Targeted inactivation of cefP by gene replacement showed that it is essential for CPC biosynthesis. The disrupted mutant is unable to synthesize cephalosporins and secretes a significant amount of IPN (isopenicillin N), indicating that the mutant is blocked in the conversion of IPN into PenN (penicillin N). The production of cephalosporin in the disrupted mutant was restored by transformation with both cefP and cefR (a regulatory gene located upstream of cefP), but not with cefP alone. Fluorescence microscopy studies with an EGFP (enhanced green fluorescent protein)\u2013SKL (Ser-Lys-Leu) protein (a peroxisomal-targeted marker) as a control showed that the red-fluorescence-labelled CefP protein co-localized in the peroxisomes with the control peroxisomal protein. In summary, CefP is a peroxisomal membrane protein probably involved in the import of IPN into the peroxisomes where it is converted into PenN by the two-component CefD1\/CefD2 protein system.<\/jats:p>","DOI":"10.1042\/bj20100827","type":"journal-article","created":{"date-parts":[[2010,9,7]],"date-time":"2010-09-07T13:32:20Z","timestamp":1283866340000},"page":"227-236","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Characterization of a novel peroxisome membrane protein essential for conversion of isopenicillin N into cephalosporin C"],"prefix":"10.1042","volume":"432","author":[{"given":"Ricardo\u00a0V.","family":"Ull\u00e1n","sequence":"first","affiliation":[{"name":"Institute of Biotechnology of Le\u00f3n (INBIOTEC), Avda Real 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Teijeira","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology of Le\u00f3n (INBIOTEC), Avda Real 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana\u00a0M.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Area of Microbiology, Faculty of Biology and Environmental Sciences, University of Le\u00f3n, Campus de Vegazana s\/n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Vaca","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology of Le\u00f3n (INBIOTEC), Avda Real 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan\u00a0F.","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology of Le\u00f3n (INBIOTEC), Avda Real 1, 24006 Le\u00f3n, Spain"},{"name":"Area of Microbiology, Faculty of Biology and Environmental Sciences, University of Le\u00f3n, Campus de Vegazana s\/n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2010,11,12]]},"reference":[{"key":"2021112217342126500_B1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s10142-009-0110-6","article-title":"Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells","volume":"9","author":"Kiel","year":"2009","journal-title":"Funct. 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