{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:25:05Z","timestamp":1792182305983,"version":"4.4.0"},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,10,15]]},"abstract":"<jats:p>On the basis of transfection experiments using a dominant-negative approach, our previous studies suggested that PKB (protein kinase B) was not involved in heart PFK-2 (6-phosphofructo2-kinase) activation by insulin. Therefore we first tested whether SGK3 (serum- and glucocorticoid-induced protein kinase 3) might be involved in this effect. Treatment of recombinant heart PFK-2 with [\u03b3-32P]ATP and SGK3 in vitro led to PFK-2 activation and phosphorylation at Ser466 and Ser483. However, in HEK-293T cells [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] co-transfected with SGK3 siRNA (small interfering RNA) and heart PFK-2, insulin-induced heart PFK-2 activation was unaffected. The involvement of PKB in heart PFK-2 activation by insulin was re-evaluated using different models: (i) hearts from transgenic mice with a muscle\/heart-specific mutation in the PDK1 (phosphoinositide-dependent protein kinase 1)-substrate-docking site injected with insulin; (ii) hearts from PKB\u03b2-deficient mice injected with insulin; (iii) freshly isolated rat cardiomyocytes and perfused hearts treated with the selective Akti-1\/2 PKB inhibitor prior to insulin treatment; and (iv) HEK-293T cells co-transfected with heart PFK-2, and PKB\u03b1\/\u03b2 siRNA or PKB\u03b1 siRNA, incubated with insulin. Together, the results indicated that SGK3 is not required for insulin-induced PFK-2 activation and that this effect is likely mediated by PKB\u03b1.<\/jats:p>","DOI":"10.1042\/bj20101089","type":"journal-article","created":{"date-parts":[[2010,8,6]],"date-time":"2010-08-06T08:22:39Z","timestamp":1281082959000},"page":"267-275","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3)"],"prefix":"10.1042","volume":"431","author":[{"given":"V\u00e9ronique","family":"Mouton","sequence":"first","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Toussaint","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"},{"name":"Cellular and Molecular Biology Unit, FUSAGx, 13 Avenue Marechal Juin, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Agn\u00e8s","family":"Gueuning","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Maisin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Havaux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain Division of Cardiology, 55 Avenue Hippocrate, CARD5550, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cossette","family":"Sanchez-Canedo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain Division of Cardiology, 55 Avenue Hippocrate, CARD5550, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain Division of Cardiology, 55 Avenue Hippocrate, CARD5550, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Dequiedt","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Unit, FUSAGx, 13 Avenue Marechal Juin, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian\u00a0A.","family":"Hemmings","sequence":"additional","affiliation":[{"name":"Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Hue","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark\u00a0H.","family":"Rider","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, 75 Avenue Hippocrate, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2010,9,28]]},"reference":[{"key":"2021112214373748700_B1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1042\/BJ20080723","article-title":"Insulin action on glucose transporters through molecular switches, tracks and tethers","volume":"413","author":"Zaid","year":"2008","journal-title":"Biochem. 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