{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T21:16:08Z","timestamp":1781817368417,"version":"3.54.5"},"reference-count":45,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,10,15]]},"abstract":"<jats:p>The hOCTN1 (human organic cation transporter 1) overexpressed in Escherichia coli and purified by Ni-chelating chromatography has been reconstituted in liposomes by detergent removal with a batch-wise procedure. The reconstitution was optimized with respect to the protein concentration, the detergent\/phospholipid ratio and the time of incubation with Amberlite XAD-4 resin. Time-dependent [14C]tetraethylammonium, [3H]carnitine or [3H]ergothioneine uptake was measured in proteoliposomes with activities ratios of 8:1.3:1 respectively. Optimal activity was found at pH\u00a08.0. The transport depended on intraliposomal ATP. [14C]tetraethylammonium transport was inhibited by several compounds. The most effective were acetyl-choline and \u03b3-butyrobetaine, followed by acetylcarnitine and tetramethylammonium. Reagents such as pyridoxal 5-phosphate, MTSES [sodium (2-sulfonatoethyl) methanethiosulfonate] and mercurials strongly inhibited the transport. From kinetic analysis of tetraethylammonium transport a Km of 0.77\u00a0mM was calculated. Acetylcholine and \u03b3-butyrobetaine behaved as competitive inhibitors of TEA (tetraethylammonium) transport with Ki values of 0.44 and 0.63\u00a0mM respectively.<\/jats:p>","DOI":"10.1042\/bj20110544","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T15:52:22Z","timestamp":1309794742000},"page":"227-233","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in<i>Escherichia coli<\/i>"],"prefix":"10.1042","volume":"439","author":[{"given":"Lorena","family":"Pochini","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Calabria, Via P. 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