{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T14:30:24Z","timestamp":1771338624691,"version":"3.50.1"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,8,15]]},"abstract":"<jats:p>Current lipidomic profiling methods rely mainly on MS to identify unknown lipids within a complex sample. We describe a new approach, involving LC\u00d7MS\/MS (liquid chromatography\u00d7tandem MS) analysis of sphingolipids based on both mass and hydrophobicity, and use this method to characterize the SM (sphingomyelin), ceramide and GalCer (galactosylceramide) content of hippocampus from AD (Alzheimer's disease) and control subjects. Using a mathematical relationship we exclude the influence of sphingolipid mass on retention time, and generate two-dimensional plots that facilitate accurate visualization and characterization of the different ceramide moieties within a given sphingolipid class, because related molecules align horizontally or vertically on the plots. Major brain GalCer species that differ in mass by only 0.04 Da were easily differentiated on the basis of their hydrophobicity. The importance of our method's capacity to define all of the major GalCer species in the brain samples is illustrated by the novel observation that the proportion of GalCer with hydroxylated fatty acids increased approximately 2-fold in the hippocampus of AD patients, compared with age- and gender-matched controls. This suggests activation of fatty acid hydroxylase in AD. Our method greatly improves the clarity of data obtained in a lipid profiling experiment and can be expanded to other lipid classes.<\/jats:p>","DOI":"10.1042\/bj20110566","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T15:52:23Z","timestamp":1309794743000},"page":"165-175","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Mass and relative elution time profiling: two-dimensional analysis of sphingolipids in Alzheimer's disease brains"],"prefix":"10.1042","volume":"438","author":[{"given":"Leila","family":"Hejazi","sequence":"first","affiliation":[{"name":"Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason\u00a0W.H.","family":"Wong","sequence":"additional","affiliation":[{"name":"Lowy Cancer Research Centre, Prince of Wales Clinical School, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danni","family":"Cheng","sequence":"additional","affiliation":[{"name":"Neuroscience Research Australia, Sydney, NSW 2031, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Proschogo","sequence":"additional","affiliation":[{"name":"Centre for Vascular Research, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diako","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"Centre for Vascular Research, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brett","family":"Garner","sequence":"additional","affiliation":[{"name":"Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony\u00a0S.","family":"Don","sequence":"additional","affiliation":[{"name":"Lowy Cancer Research Centre, Prince of Wales Clinical School, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2011,7,27]]},"reference":[{"key":"2021112215184284500_B1","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1194\/jlr.R800073-JLR200","article-title":"Sphingolipidomics: a valuable tool for understanding the roles of sphingolipids in biology and disease","volume":"50","author":"Merrill","year":"2009","journal-title":"J. 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