{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:57:14Z","timestamp":1791291434969,"version":"4.3.0"},"reference-count":29,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,10,1]]},"abstract":"<jats:p>TIR (Toll\/interleukin-1 receptor) domain-containing proteins play a crucial role in innate immunity in eukaryotes. Brucella is a highly infectious intracellular bacterium that encodes a TIR domain protein (TcpB) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. TcpB inhibits NF-\u03baB (nuclear factor \u03baB) activation and pro-inflammatory cytokine secretions mediated by TLR (Toll-like receptor) 2 and TLR4. In the present study, we have demonstrated that TcpB modulates microtubule dynamics by acting as a stabilization factor. TcpB increased the rate of nucleation as well as the polymerization phases of microtubule formation in a similar manner to paclitaxel. TcpB could efficiently inhibit nocodazole- or cold-induced microtubule disassembly. Microtubule stabilization by TcpB is attributed to the BB-loop region of the TIR domain, and a point mutation affected the microtubule stabilization as well as the TLR-suppression properties of TcpB.<\/jats:p>","DOI":"10.1042\/bj20110577","type":"journal-article","created":{"date-parts":[[2011,7,4]],"date-time":"2011-07-04T15:52:49Z","timestamp":1309794769000},"page":"79-83","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen <i>Brucella melitensis<\/i>"],"prefix":"10.1042","volume":"439","author":[{"given":"Girish\u00a0K.","family":"Radhakrishnan","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, 1656 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome\u00a0S.","family":"Harms","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, 1656 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary\u00a0A.","family":"Splitter","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, 1656 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2011,9,14]]},"reference":[{"key":"2021112214515642100_B1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/0-387-24180-9_2","article-title":"Toll-like receptors: linking innate and adaptive immunity","volume":"560","author":"Pasare","year":"2005","journal-title":"Adv. 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