{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:04:14Z","timestamp":1789650254650,"version":"4.0.0"},"reference-count":52,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,4,1]]},"abstract":"<jats:p>n\u22123 PUFA (polyunsaturated fatty acids), i.e. DHA (docosahexaenoic acid), found in fish oil, exhibit anti-inflammatory properties; however, the molecular mechanisms remain unclear. Since PtdIns(4,5)P2 resides in raft domains and DHA can alter the size of rafts, we hypothesized that PtdIns(4,5)P2 and downstream actin remodelling are perturbed by the incorporation of n\u22123 PUFA into membranes, resulting in suppressed T-cell activation. CD4+ T-cells isolated from Fat-1 transgenic mice (membranes enriched in n\u22123 PUFA) exhibited a 50% decrease in PtdIns(4,5)P2. Upon activation by plate-bound anti-CD3\/anti-CD28 or PMA\/ionomycin, Fat-1 CD4+ T-cells failed to metabolize PtdIns(4,5)P2. Furthermore, actin remodelling failed to initiate in Fat-1 CD4+ T-cells upon stimulation; however, the defect was reversed by incubation with exogenous PtdIns(4,5)P2. When Fat-1 CD4+ T-cells were stimulated with anti-CD3\/anti-CD28-coated beads, WASP (Wiskott\u2013Aldrich syndrome protein) failed to translocate to the immunological synapse. The suppressive phenotype, consisting of defects in PtdIns(4,5)P2 metabolism and actin remodelling, were recapitulated in CD4+ T-cells isolated from mice fed on a 4% DHA triacylglycerol-enriched diet. Collectively, these data demonstrate that n\u22123 PUFA, such as DHA, alter PtdIns(4,5)P2 in CD4+ T-cells, thereby suppressing the recruitment of WASP to the immunological synapse, and impairing actin remodelling in CD4+ T-cells.<\/jats:p>","DOI":"10.1042\/bj20111589","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T10:16:09Z","timestamp":1326968169000},"page":"27-37","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["<i>n<\/i>\u22123 polyunsaturated fatty acids suppress phosphatidylinositol 4,5-bisphosphate-dependent actin remodelling during CD4+ T-cell activation"],"prefix":"10.1042","volume":"443","author":[{"given":"Tim\u00a0Y.","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer\u00a0M.","family":"Monk","sequence":"additional","affiliation":[{"name":"Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Texas AgriLife Vegetable Crop Improvement Center, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Yi","family":"Fan","sequence":"additional","affiliation":[{"name":"Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Texas AgriLife Vegetable Crop Improvement Center, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rola","family":"Barhoumi","sequence":"additional","affiliation":[{"name":"Center for Environmental and Rural Health, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u00a0Q.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Wake Forest University, Winston-Salem, NC, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo\u00a0M.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David\u00a0N.","family":"McMurray","sequence":"additional","affiliation":[{"name":"Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Texas AgriLife Vegetable Crop Improvement Center, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Center for Environmental and Rural Health, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Faculty of Nutrition, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert\u00a0S.","family":"Chapkin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Texas AgriLife Vegetable Crop Improvement Center, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Center for Environmental and Rural Health, Texas A&M University, College Station, Texas, U.S.A."},{"name":"Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2012,3,14]]},"reference":[{"key":"2021112217262230800_B1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.molmed.2009.03.002","article-title":"CD4 T-cell differentiation and inflammatory bowel disease","volume":"15","author":"Zenewicz","year":"2009","journal-title":"Trends Mol. 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