{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:01:00Z","timestamp":1791295260586,"version":"4.1.0"},"reference-count":44,"publisher":"Portland Press Ltd.","issue":"3","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":2708,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,3,15]]},"abstract":"<jats:p>PKD (protein kinase D) 2 is a serine\/threonine kinase activated by diacylglycerol in response to engagement of antigen receptors in lymphocytes. To explore PKD2 regulation and function in TCR (T-cell antigen receptor) signal transduction we expressed TCR complexes with fixed affinity for self antigens in the T-cells of PKD2-null mice or mice deficient in PKD2 catalytic activity. We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engagement of \u03b1\/\u03b2 TCR complexes in vivo. Strikingly, PKD2 loss caused increases in thymic output, lymphadenopathy and splenomegaly in TCR transgenic mice. The precise magnitude and timing of PKD2 activation during T-cell development is thus critical to regulate thymic homoeostasis. PKD2-null T-cells that exit the thymus have a normal transcriptome, but show a limited and abnormal transcriptional response to antigen. Transcriptional profiling reveals the full consequences of PKD2 loss and maps in detail the selective, but critical, function for PKD2 in signalling by \u03b1\/\u03b2 mature TCR complexes in peripheral T-cells.<\/jats:p>","DOI":"10.1042\/bj20111700","type":"journal-article","created":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T09:50:30Z","timestamp":1326275430000},"page":"649-659","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells"],"prefix":"10.1042","volume":"442","author":[{"given":"Maria\u00a0N.","family":"Navarro","sequence":"first","affiliation":[{"name":"The College of Life Sciences, Division of Cell Signalling and Immunology, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda\u00a0V.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"The College of Life Sciences, Division of Cell Signalling and Immunology, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Feijoo-Carnero","sequence":"additional","affiliation":[{"name":"The College of Life Sciences, Division of Cell Signalling and Immunology, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Clarke","sequence":"additional","affiliation":[{"name":"The College of Life Sciences, Division of Cell Signalling and Immunology, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon\u00a0A.","family":"Matthews","sequence":"additional","affiliation":[{"name":"The Medical Research Institute, Ninewells Hospital and Medical School, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doreen\u00a0A.","family":"Cantrell","sequence":"additional","affiliation":[{"name":"The College of Life Sciences, Division of Cell Signalling and Immunology, The University of Dundee, Dundee, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2012,2,24]]},"reference":[{"key":"2021112217301930200_B1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1600-065X.2008.00745.x","article-title":"T-cell receptor proximal signaling via the Src-family kinases, Lck and Fyn, influences T-cell activation, differentiation, and tolerance","volume":"228","author":"Salmond","year":"2009","journal-title":"Immunol. 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