{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:40Z","timestamp":1791338260249,"version":"4.3.1"},"reference-count":45,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,2,15]]},"abstract":"<jats:p>The retromer complex is a conserved endosomal protein sorting complex that sorts membrane proteins into nascent endosomal tubules. The recognition of membrane proteins is mediated by the cargo-selective retromer complex, a stable trimer of the Vps35 (vacuolar protein sorting 35), Vps29 and Vps26 proteins. We have recently reported that the cargo-selective retromer complex associates with the WASH (Wiskott\u2013Aldrich syndrome homologue) complex, a multimeric protein complex that regulates tubule dynamics at endosomes. In the present study, we show that the retromer\u2013WASH complex interaction occurs through the long unstructured \u2018tail\u2019 domain of the WASH complex\u2013Fam21 protein binding to Vps35, an interaction that is necessary and sufficient to target the WASH complex to endosomes. The Fam21-tail also binds to FKBP15 (FK506-binding protein 15), a protein associated with ulcerative colitis, to mediate the membrane association of FKBP15. Elevated Fam21-tail expression inhibits the association of the WASH complex with retromer, resulting in increased cytoplasmic WASH complex. Additionally, overexpression of the Fam21-tail results in cell-spreading defects, implicating the activity of the WASH complex in regulating the mobilization of membrane into the endosome-to-cell surface pathway.<\/jats:p>","DOI":"10.1042\/bj20111761","type":"journal-article","created":{"date-parts":[[2011,11,10]],"date-time":"2011-11-10T09:23:03Z","timestamp":1320916983000},"page":"209-220","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":238,"title":["Recruitment of the endosomal WASH complex is mediated by the extended \u2018tail\u2019 of Fam21 binding to the retromer protein Vps35"],"prefix":"10.1042","volume":"442","author":[{"given":"Michael\u00a0E.","family":"Harbour","sequence":"first","affiliation":[{"name":"Cambridge Institute for Medical Research (CIMR)\/Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia\u00a0Y.","family":"Breusegem","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research (CIMR)\/Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew\u00a0N.\u00a0J.","family":"Seaman","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research (CIMR)\/Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2012,1,27]]},"reference":[{"key":"2021112217273671600_B1","first-page":"99","article-title":"The early endosome: a busy sorting station for proteins at the crossroads","volume":"25","author":"Jovic","year":"2010","journal-title":"Histol. Histopathol."},{"key":"2021112217273671600_B2","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1038\/ncb1657","article-title":"Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts","volume":"9","author":"Balasubramanian","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"2021112217273671600_B3","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1242\/jcs.03383","article-title":"EHD1 regulates \u03b21 integrin endosomal transport: effects on focal adhesions, cell spreading and migration","volume":"120","author":"Jovi\u0107","year":"2007","journal-title":"J. Cell Sci."},{"key":"2021112217273671600_B4","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1016\/j.yexcr.2011.04.016","article-title":"VAMP3 regulates podosome organisation in macrophages and together with Stx4\/SNAP23 mediates adhesion, cell spreading and persistent migration","volume":"317","author":"Veale","year":"2011","journal-title":"Exp. Cell Res."},{"key":"2021112217273671600_B5","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/j.1471-4159.2010.06976.x","article-title":"Niemann\u2013Pick type\u00a0C disease: molecular mechanisms and potential therapeutic approaches","volume":"116","author":"Rosenbaum","year":"2011","journal-title":"J. Neurochem."},{"key":"2021112217273671600_B6","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/S1474-4422(08)70258-8","article-title":"Hereditary spastic paraplegia: clinical features and pathogenetic mechanisms","volume":"7","author":"Salinas","year":"2008","journal-title":"Lancet Neurol."},{"key":"2021112217273671600_B7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nrn2946","article-title":"Hereditary spastic paraplegias: membrane traffic and the motor pathway","volume":"12","author":"Blackstone","year":"2011","journal-title":"Nat. Rev. Neurosci."},{"key":"2021112217273671600_B8","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1111\/j.1600-0854.2011.01188.x","article-title":"Physiology and pathology of endosome-to-Golgi retrograde sorting","volume":"12","author":"Burd","year":"2011","journal-title":"Traffic"},{"key":"2021112217273671600_B9","doi-asserted-by":"crossref","first-page":"4715","DOI":"10.1021\/bi100397s","article-title":"Regulation and isoform function of the V-ATPases","volume":"49","author":"Toei","year":"2010","journal-title":"Biochemistry"},{"key":"2021112217273671600_B10","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.ceb.2008.03.015","article-title":"The V-type H+-ATPase in vesicular trafficking: targeting, regulation and function","volume":"20","author":"Marshansky","year":"2008","journal-title":"Curr. Opin. Cell Biol."},{"key":"2021112217273671600_B11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1083\/jcb.200312055","article-title":"Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor","volume":"165","author":"Arighi","year":"2004","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B12","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.200312034","article-title":"Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer","volume":"165","author":"Seaman","year":"2004","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B13","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.bbrc.2007.12.015","article-title":"AP-1 and retromer play opposite roles in the trafficking of sortilin between the Golgi apparatus and the lysosomes","volume":"366","author":"Canuel","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021112217273671600_B14","doi-asserted-by":"crossref","first-page":"6842","DOI":"10.1128\/MCB.00815-07","article-title":"Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA","volume":"27","author":"Nielsen","year":"2007","journal-title":"Mol. Cell Biol."},{"key":"2021112217273671600_B15","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1242\/jcs.060574","article-title":"Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1","volume":"123","author":"Tabuchi","year":"2010","journal-title":"J. Cell Sci."},{"key":"2021112217273671600_B16","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tcb.2004.12.004","article-title":"Recycle your receptors with retromer","volume":"15","author":"Seaman","year":"2005","journal-title":"Trends Cell Biol."},{"key":"2021112217273671600_B17","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1111\/j.1600-0854.2008.00777.x","article-title":"The structure and function of the retromer protein complex","volume":"9","author":"Collins","year":"2008","journal-title":"Traffic"},{"key":"2021112217273671600_B18","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1083\/jcb.200804048","article-title":"Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7","volume":"183","author":"Rojas","year":"2008","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B19","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1242\/jcs.048686","article-title":"Membrane recruitment of the cargo-selective retromer subcomplex is catalysed by the small GTPase Rab7 and inhibited by the Rab-GAP TBC1D5","volume":"122","author":"Seaman","year":"2009","journal-title":"J. Cell Sci."},{"key":"2021112217273671600_B20","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1083\/jcb.151.2.297","article-title":"Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p","volume":"151","author":"Nothwehr","year":"2000","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B21","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/nsmb1103","article-title":"The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain","volume":"13","author":"Shi","year":"2006","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2021112217273671600_B22","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1111\/j.1600-0854.2007.00688.x","article-title":"Structure of Vps26B and mapping of its interaction with the retromer protein complex","volume":"9","author":"Collins","year":"2008","journal-title":"Traffic"},{"key":"2021112217273671600_B23","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.advenzreg.2009.10.002","article-title":"The retromer complex","volume":"50","author":"Attar","year":"2010","journal-title":"Adv. Enzyme Regul."},{"key":"2021112217273671600_B24","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1016\/j.cub.2004.09.077","article-title":"Sorting nexin-1 mediates tubular endosome-to-TGN transport through coincidence sensing of high-curvature membranes and 3-phosphoinositides","volume":"14","author":"Carlton","year":"2004","journal-title":"Curr. Biol."},{"key":"2021112217273671600_B25","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1242\/jcs.071472","article-title":"The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics","volume":"123","author":"Harbour","year":"2010","journal-title":"J. Cell Sci."},{"key":"2021112217273671600_B26","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1016\/j.yexcr.2008.12.004","article-title":"WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics","volume":"15","author":"Viklund","year":"2009","journal-title":"Exp. Cell Res."},{"key":"2021112217273671600_B27","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.bbrc.2006.05.113","article-title":"FKBP133: a novel mouse FK506-binding protein homolog alters growth cone morphology","volume":"346","author":"Nakajima","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021112217273671600_B28","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.devcel.2009.09.009","article-title":"A FAM21-containing WASH complex regulates retromer-dependent sorting","volume":"17","author":"Gomez","year":"2009","journal-title":"Dev. Cell"},{"key":"2021112217273671600_B29","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.devcel.2009.09.010","article-title":"The Arp2\/3 activator WASH controls the fission of endosomes through a large multiprotein complex","volume":"17","author":"Derivery","year":"2009","journal-title":"Dev. Cell"},{"key":"2021112217273671600_B30","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1083\/jcb.201009119","article-title":"Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis","volume":"193","author":"Carnell","year":"2011","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B31","doi-asserted-by":"crossref","first-page":"10442","DOI":"10.1073\/pnas.0913293107","article-title":"WASH and WAVE actin regulators of the Wiskott\u2013Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes","volume":"107","author":"Jia","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021112217273671600_B32","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nsmb.1792","article-title":"Structural characterization of a capping protein interaction motif defines a family of actin filament regulators","volume":"17","author":"Hernandez-Valladares","year":"2010","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2021112217273671600_B33","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1002\/j.1460-2075.1987.tb02659.x","article-title":"Functional equivalents of interferon-mediated signals needed for induction of an mRNA can be generated by double-stranded RNA and growth factors","volume":"6","author":"Tiwari","year":"1987","journal-title":"EMBO J."},{"key":"2021112217273671600_B34","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1083\/jcb.200609161","article-title":"Grd19\/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling","volume":"177","author":"Strochlic","year":"2007","journal-title":"J. Cell Biol."},{"key":"2021112217273671600_B35","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1038\/ncb2281","article-title":"A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion","volume":"13","author":"Harterink","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"2021112217273671600_B36","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1038\/nature06216","article-title":"Functional architecture of the retromer cargo-recognition complex","volume":"449","author":"Hierro","year":"2007","journal-title":"Nature"},{"key":"2021112217273671600_B37","doi-asserted-by":"crossref","first-page":"4367","DOI":"10.1242\/jcs.012336","article-title":"Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation","volume":"120","author":"Korolchuk","year":"2007","journal-title":"J. Cell Sci."},{"key":"2021112217273671600_B38","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1002\/j.1460-2075.1994.tb06514.x","article-title":"Retrieval of TGN proteins from the cell surface requires endosomal acidification","volume":"13","author":"Chapman","year":"1994","journal-title":"EMBO J."},{"key":"2021112217273671600_B39","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cell.2010.01.032","article-title":"The genome of Naegleria gruberi illuminates early eukaryotic versatility","volume":"140","author":"Fritz-Laylin","year":"2010","journal-title":"Cell"},{"key":"2021112217273671600_B40","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1101\/gr.2659105","article-title":"Protein interaction mapping: a Drosophila case study","volume":"15","author":"Formstecher","year":"2005","journal-title":"Genome Res."},{"key":"2021112217273671600_B41","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/EC.00322-06","article-title":"Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium","volume":"6","author":"Damer","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"2021112217273671600_B42","doi-asserted-by":"crossref","first-page":"10048","DOI":"10.1074\/jbc.M212632200","article-title":"Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif","volume":"278","author":"Tomsig","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2021112217273671600_B43","article-title":"CCDC22: a novel candidate gene for syndromic X-linked intellectual disability","author":"Voineagu","year":"2011","journal-title":"Mol. Psychiatry"},{"key":"2021112217273671600_B44","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/ncb2252","article-title":"SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors","volume":"13","author":"Temkin","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"2021112217273671600_B45","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1042\/BJ20070555","article-title":"Identification of a conserved motif required for Vps35p\/Vps26p interaction and assembly of the retromer complex","volume":"408","author":"Gokool","year":"2007","journal-title":"Biochem. J."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/442\/1\/209\/639769\/bj4420209.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/442\/1\/209\/639769\/bj4420209.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T01:15:45Z","timestamp":1637630145000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/442\/1\/209\/80169\/Recruitment-of-the-endosomal-WASH-complex-is"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,27]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20111761","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,27]]}}}