{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T09:02:31Z","timestamp":1693558951728},"reference-count":38,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,7,1]]},"abstract":"<jats:p>The closely related pathogenic Neisseria species N. meningitidis and N. gonorrhoeae are able to respire in the absence of oxygen, using nitrite as an alternative electron acceptor. aniA (copper-containing nitrite reductase) is tightly regulated by four transcriptional regulators: FNR (fumarate and nitrate reductase), NarP, FUR (Ferric uptake regulator) and NsrR. The four regulators control expression of aniA in N. meningitidis by binding to specific and distinct regions of the promoter. We show in the present study that FUR and NarP are both required for the induction of expression of aniA in N. meningitidis, and that they bind adjacent to one another in a non-co-operative manner. Activation via FUR\/NarP is dependent on their topological arrangement relative to the RNA polymerase-binding site. Analysis of the sequence of the aniA promoters from multiple N. meningitidis and N. gonorrhoeae strains indicates that there are species-specific single nucleotide polymorphisms, in regions predicted to be important for regulator binding. These sequence differences alter both the in vitro DNA binding and the promoter activation in intact cells by key activators FNR (oxygen sensor) and NarP (which is activated by nitrite in N. meningitidis). The weak relative binding of FNR to the N. gonorrhoeae aniA promoter (compared to N. meningitidis) is compensated for by a higher affinity of the gonococcal aniA promoter for NarP. Despite containing nearly identical genes for catalysing and regulating denitrification, variations in the promoter for the aniA gene appear to have been selected to enable the two pathogens to tune differentially their responses to environmental variables during the aerobic\u2013anaerobic switch.<\/jats:p>","DOI":"10.1042\/bj20111984","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T09:03:06Z","timestamp":1334826186000},"page":"69-79","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Neisseria meningitidis<\/i> and <i>Neisseria gonorrhoeae<\/i> are differently adapted in the regulation of denitrification: single nucleotide polymorphisms that enable species-specific tuning of the aerobic\u2013anaerobic switch"],"prefix":"10.1042","volume":"445","author":[{"given":"James","family":"Edwards","sequence":"first","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Quinn","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karyn-Anne","family":"Rowbottom","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u00a0L.","family":"Whittingham","sequence":"additional","affiliation":[{"name":"York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie\u00a0J.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James\u00a0W.\u00a0B.","family":"Moir","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2012,6,15]]},"reference":[{"key":"2021112215232258200_B1","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1128\/JB.184.11.2987-2993.2002","article-title":"Nitric oxide metabolism in Neisseria meningitidis","volume":"184","author":"Anjum","year":"2002","journal-title":"J. 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