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Incubation of freshly isolated rat hepatocytes with the pharmacological AMPK activators AICA riboside (5-aminoimidazole-4-carboxamide-1-\u03b2-D-ribofuranoside) or A769662 led to persistent GYS inactivation and Ser7 phosphorylation, whereas inactivation by glucagon treatment was transient. In hepatocytes from mice harbouring a liver-specific deletion of the AMPK catalytic \u03b11\/\u03b12 subunits, GYS2 inactivation by AICA riboside and A769662 was blunted, whereas inactivation by glucagon was unaffected. The results suggest that GYS inactivation by AMPK activators in hepatocytes is due to GYS2 Ser7 phosphorylation.<\/jats:p>","DOI":"10.1042\/bj20112026","type":"journal-article","created":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T12:36:44Z","timestamp":1326371804000},"page":"193-203","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":122,"title":["AMP-activated protein kinase phosphorylates and inactivates liver glycogen synthase"],"prefix":"10.1042","volume":"443","author":[{"given":"Laurent","family":"Bultot","sequence":"first","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Guigas","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Leiden University Medical Center, Postzone S1-P, Postbus 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Von Wilamowitz-Moellendorff","sequence":"additional","affiliation":[{"name":"MRC Protein Phosphorylation Unit, Sir James Black Centre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Maisin","sequence":"additional","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nusrat","family":"Hussain","sequence":"additional","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Beullens","sequence":"additional","affiliation":[{"name":"Laboratory of Biosignaling and Therapeutics, Department of Molecular Cell Biology, University of Leuven, B-3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan\u00a0J.","family":"Guinovart","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Foretz","sequence":"additional","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR8104, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris 75014, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Viollet","sequence":"additional","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR8104, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris 75014, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"MRC Protein Phosphorylation Unit, Sir James Black Centre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Hue","sequence":"additional","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark\u00a0H.","family":"Rider","sequence":"additional","affiliation":[{"name":"de Duve Institute, Universit\u00e9 catholique de Louvain, Avenue Hippocrate 75, bte B1.74.02, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2012,3,14]]},"reference":[{"key":"2021112217281767700_B1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2174\/1566524024605761","article-title":"Glycogen and its metabolism","volume":"2","author":"Roach","year":"2002","journal-title":"Curr. 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