{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:06:47Z","timestamp":1761919607660},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,11,15]]},"abstract":"<jats:p>The Scar (suppressor of cAMP receptor)\/WAVE [WASP (Wiskott\u2013Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2\/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar\/WAVE isoforms, which show some tissue-specific expression, but their functions have not been differentiated. In the present study we show that depletion of Scar\/WAVE3\u00a0in the mammalian breast cancer cells MDA-MB-231 results in larger and less dynamic lamellipodia. Scar\/WAVE3-depleted cells move more slowly but more persistently on a two-dimensional matrix and they typically only show one lamellipod. However, Scar\/WAVE3 appears to have no role in driving invasiveness in a three-dimensional Matrigel\u2122 invasion assay or a three-dimensional collagen invasion assay, suggesting that lamellipodial persistence as seen in two-dimensions is not crucial in three-dimensional environments.<\/jats:p>","DOI":"10.1042\/bj20112206","type":"journal-article","created":{"date-parts":[[2012,8,22]],"date-time":"2012-08-22T08:11:49Z","timestamp":1345623109000},"page":"35-42","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Scar\/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions"],"prefix":"10.1042","volume":"448","author":[{"given":"Heather\u00a0J.","family":"Spence","sequence":"first","affiliation":[{"name":"The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Timpson","sequence":"additional","affiliation":[{"name":"The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u00a0Ran","family":"Tang","sequence":"additional","affiliation":[{"name":"The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert\u00a0H.","family":"Insall","sequence":"additional","affiliation":[{"name":"The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura\u00a0M.","family":"Machesky","sequence":"additional","affiliation":[{"name":"The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2012,10,18]]},"reference":[{"key":"2021112215281981400_B1","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/nature00859","article-title":"Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck","volume":"418","author":"Eden","year":"2002","journal-title":"Nature"},{"key":"2021112215281981400_B2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1471-2121-6-11","article-title":"Inclusion of Scar\/WAVE3\u00a0in a similar complex to Scar\/WAVE1 and 2","volume":"6","author":"Stovold","year":"2005","journal-title":"BMC Cell Biol."},{"key":"2021112215281981400_B3","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/S0960-9822(98)00015-3","article-title":"Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2\/3 complex","volume":"8","author":"Machesky","year":"1998","journal-title":"Curr. 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