{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:12:43Z","timestamp":1785402763211,"version":"3.56.0"},"reference-count":49,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p>The stress response of eukaryotic cells often causes an attenuation of bulk translation activity and the accumulation of non-translating mRNAs into cytoplasmic mRNP (messenger ribonucleoprotein) granules termed cytoplasmic P-bodies (processing bodies) and SGs (stress granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca2+ level in Saccharomyces cerevisiae. Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused an attenuation of translation initiation, albeit to a lesser extent than glucose depletion. P-body formation was also induced by hydrochloric acid and sulfuric acid. However, lactic acid in SD (synthetic dextrose) medium with a pH greater than 3.0, propionic acid and acetic acid did not induce P-body formation. The results of the present study suggest that the assembly of yeast P-bodies can be induced by external conditions with a low pH and the threshold was around pH\u00a02.5. The P-body formation upon acidic stress required Scd6 (suppressor of clathrin deficiency 6), a component of P-bodies, indicating that P-bodies induced by acidic stress have rules of assembly different from those induced by glucose deprivation or high Ca2+ levels.<\/jats:p>","DOI":"10.1042\/bj20120583","type":"journal-article","created":{"date-parts":[[2012,6,13]],"date-time":"2012-06-13T08:38:56Z","timestamp":1339576736000},"page":"225-233","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in<i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1042","volume":"446","author":[{"given":"Aya","family":"Iwaki","sequence":"first","affiliation":[{"name":"Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Izawa","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2012,8,14]]},"reference":[{"key":"2021112215294663700_B1","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/nrm2694","article-title":"RNA granules: post-transcriptional and epigenetic modulators of gene expression","volume":"10","author":"Anderson","year":"2009","journal-title":"Nat. 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