{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:30:57Z","timestamp":1791048657100,"version":"4.1.0"},"reference-count":50,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,5,15]]},"abstract":"<jats:p>The NXF (nuclear export factor) family members are implicated in the transport of mRNA from the nucleus to the cytoplasm. Recently, some members of the NXF family have been reported to play divergent functional roles, such as post-transcriptional regulation, translational control, regulation of mRNA stability and trafficking. However, little is known about the roles of NXF3 in spermatogenesis. In the present study, we found that mouse NXF3, specifically expressed in principal cells in segment II of the caput epididymis, as well as Sertoli cells in the mouse testis, was required to mediate TGF-\u03b2 (transforming growth factor \u03b2)-induced down-regulation of Tgfb3\/TGF-\u03b23 mRNA expression and protein secretion in Sertoli cells. In addition, NXF3 was also involved in TGF-\u03b2-induced transcriptional regulation of other genes associated with Sertoli cell maturation and the restructuring of the Sertoli cell BTB (blood\u2013testis barrier), such as Gata1 (GATA-binding protein 1), Wt1 (Wilms's tumour homologue 1), Cldn11 (claudin11) and Cdkn1a (cyclin-dependent kinase inhibitor 1A or p21Cip1). The transcriptional regulation of NXF3 was mediated through physical interaction with STRAP (serine\/threonine kinase receptor-associated protein), where NXF3 inhibited the complex formation among Smad7, STRAP and activated type\u00a0I TGF-\u03b2 receptor. Taken together, our data provide mechanistic insights into the roles of NXF3 in TGF-\u03b2-mediated expression of Tgfb3 and other genes. NXF3 may be implicated in Sertoli cell maturation and the extensive restructuring of the Sertoli cell BTB.<\/jats:p>","DOI":"10.1042\/bj20121006","type":"journal-article","created":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T09:01:43Z","timestamp":1361869303000},"page":"67-78","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Nuclear export factor 3 is involved in regulating the expression of TGF-\u03b23 in an mRNA export activity-independent manner in mouse Sertoli cells"],"prefix":"10.1042","volume":"452","author":[{"given":"Yimeng","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guishuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China, and Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2013,4,25]]},"reference":[{"key":"2021112215385569400_B1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1095\/biolreprod52.2.211","article-title":"Interactions between germ cells and Sertoli cells in the testis","volume":"52","author":"Griswold","year":"1995","journal-title":"Biol. 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