{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T02:10:01Z","timestamp":1775182201896,"version":"3.50.1"},"reference-count":33,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,4,15]]},"abstract":"<jats:p>Gene deletion studies in mice have revealed critical roles for IL (interleukin)-4 and -13\u00a0in asthma development, with the latter controlling lung airways resistance and mucus secretion. We have now developed human neutralizing monoclonal antibodies against human IL-13R\u03b11 (IL-13 receptor \u03b11) subunit that prevent activation of the receptor complex by both IL-4 and IL-13. We describe the crystal structures of the Fab fragment of antibody 10G5H6 alone and in complex with D3 (ectodomain 3) of IL-13R\u03b11. Although the structure showed significant domain swapping within a D3 dimer, we showed that Arg230, Phe233, Tyr250, Gln252 and Leu293 in each D3 monomer and Ser32, Asn102 and Trp103 in 10G5H6 Fab are the key interacting residues at the interface of the 10G5H6 Fab\u2013D3 complex. One of the most striking contacts is the insertion of the ligand-contacting residue Leu293 of D3 into a deep pocket on the surface of 10G5H6 Fab, and this appears to be a central determinant of the high binding affinity and neutralizing activity of the antibody.<\/jats:p>","DOI":"10.1042\/bj20121819","type":"journal-article","created":{"date-parts":[[2013,2,6]],"date-time":"2013-02-06T09:38:50Z","timestamp":1360143530000},"page":"165-175","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Production of a human neutralizing monoclonal antibody and its crystal structure in complex with ectodomain 3 of the interleukin-13 receptor \u03b11"],"prefix":"10.1042","volume":"451","author":[{"given":"Nicholas\u00a0T.","family":"Redpath","sequence":"first","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia"},{"name":"Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibin","family":"Xu","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia"},{"name":"Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas\u00a0J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"CSL Limited, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis\u00a0J.","family":"Fabri","sequence":"additional","affiliation":[{"name":"CSL Limited, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Baca","sequence":"additional","affiliation":[{"name":"CSL Limited, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arna\u00a0E.","family":"Andrews","sequence":"additional","affiliation":[{"name":"CSL Limited, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hal","family":"Braley","sequence":"additional","affiliation":[{"name":"CSL Limited, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lu","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl","family":"Ireland","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin\u00a0E.","family":"Ernst","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Woods","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail","family":"Forrest","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"An","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis\u00a0M.","family":"Zaller","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William\u00a0R.","family":"Strohl","sequence":"additional","affiliation":[{"name":"Merck Research Laboratories, West Point, PA 19486, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy\u00a0S.","family":"Luo","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter\u00a0E.","family":"Czabotar","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia"},{"name":"Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas\u00a0P. 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