{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:14:45Z","timestamp":1791195285851,"version":"4.1.0"},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,5,15]]},"abstract":"<jats:p>Homozygous mutations in BSCL2 (Berardinelli\u2013Seip congenital lipodystrophy)\/seipin cause CGL2 (congenital generalized lipodystrophy type\u00a02). Recent data suggest that seipin regulates LD (lipid droplet) dynamics and adipocyte differentiation, but whether these roles are mechanistically linked remains unclear. To understand how seipin regulates these processes, we investigated the evolutionary changes of seipin orthologues, and studied individual domains in regulating lipid accumulation in non-adipocytes and adipocytes. Mammalian seipins comprise at least two distinct functional domains, a conserved core sequence and an evolutionarily acquired C-terminus. Despite its requirement for adipocyte formation, seipin overexpression inhibited oleate-induced LD formation and accumulation in nonadipocytes, which was mediated by the core sequence. In contrast, seipin overexpression did not inhibit LD accumulation during adipocyte differentiation or the adipogenic process in 3T3-L1 cells. However, adipogenesis and LD accumulation were impaired in 3T3-L1 cells expressing a seipin mutant lacking the C-terminus. Furthermore, expression of the same mutant without the C-terminus failed to rescue the adipogenic defects in seipin-knockdown cells, demonstrating the importance of the C-terminus for seipin's function in adipocyte development. We propose that seipin is involved in lipid homoeostasis by restricting lipogenesis and LD accumulation in non-adipocytes, while promoting adipogenesis to accommodate excess energy storage.<\/jats:p>","DOI":"10.1042\/bj20121870","type":"journal-article","created":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T10:43:07Z","timestamp":1362566587000},"page":"37-44","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Seipin differentially regulates lipogenesis and adipogenesis through a conserved core sequence and an evolutionarily acquired C-terminus"],"prefix":"10.1042","volume":"452","author":[{"given":"Wulin","family":"Yang","sequence":"first","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shermaine","family":"Thein","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxiang","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Xu","sequence":"additional","affiliation":[{"name":"Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research (A*STAR), 30 Medical Drive, Singapore 117609"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byrappa","family":"Venkatesh","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138673"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeki","family":"Sugii","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"},{"name":"Duke-NUS Graduate Medical School, Singapore 169857"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George\u00a0K.","family":"Radda","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Han","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, 11 Biopolis Way, Singapore 138667"},{"name":"Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research (A*STAR), 30 Medical Drive, Singapore 117609"},{"name":"Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138673"},{"name":"Duke-NUS Graduate Medical School, Singapore 169857"},{"name":"Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2013,4,25]]},"reference":[{"key":"2021112215391106400_B1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1651-2227.1996.tb14262.x","article-title":"Generalized lipodystrophy, congenital and acquired (lipoatrophy)","volume":"413","author":"Seip","year":"1996","journal-title":"Acta Paediatr. 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