{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T04:23:49Z","timestamp":1649219029807},"reference-count":25,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:p>Human EMCs (extraskeletal myxoid chondrosarcomas) are soft tissue tumours characterized by specific chromosomal abnormalities. Recently, a proportion of EMCs were found to harbour a characteristic translocation, t(3;9)(q11-12;q22), involving the TFG (TRK-fused gene) at 3q11-12 and the TEC (translocated in extraskeletal chondrosarcoma) gene at 9q22. The present study used both in\u00a0vitro and in\u00a0vivo systems to show that the TFG\u2013TEC protein self-associates, and that this is dependent upon the CC (coiled-coil) domain (amino acids 97\u2013124), the AF1 (activation function 1) domain (amino acids 275\u2013562) and the DBD (DNA-binding domain) (amino acids 563\u2013655). The TFG\u2013TEC protein also associated with a mutant NLS-TFG\u2013TEC (AAAA) protein, which harbours mutations in the NLS (nuclear localization signal). Subcellular localization assays showed that the NLS mutant TFG\u2013TEC (AAAA) protein interfered with the nuclear localization of wild-type TFG\u2013TEC. Most importantly, the mutant protein inhibited TFG\u2013TEC-mediated transcriptional activation in\u00a0vivo. Thus mutations in the TFG\u2013TEC NLS yield a dominant-negative protein. These results show that the biological functions of the TFG-TEC oncogene can be modulated by a dominant-negative mutant.<\/jats:p>","DOI":"10.1042\/bj20130486","type":"journal-article","created":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T11:16:19Z","timestamp":1380539779000},"page":"361-372","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A TFG\u2013TEC nuclear localization mutant forms complexes with the wild-type TFG\u2013TEC oncoprotein and suppresses its activity"],"prefix":"10.1042","volume":"456","author":[{"given":"Bobae","family":"Lim","sequence":"first","affiliation":[{"name":"Laboratory of Molecular and Cellular Biology, Department of Life Sciences, Sogang University, Seoul 121-742, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ah-young","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biology, Department of Life Sciences, Sogang University, Seoul 121-742, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee\u00a0Jung","family":"Jun","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biology, Department of Life Sciences, Sogang University, Seoul 121-742, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jungho","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Biology, Department of Life Sciences, Sogang University, Seoul 121-742, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2013,11,22]]},"reference":[{"key":"2021112215491924300_B1","first-page":"229","article-title":"Fusion of the EWS gene to CHN, a member of the steroid\/thyroid receptor gene superfamily, in a human myxoid chondrosarcoma","volume":"12","author":"Clark","year":"1996","journal-title":"Oncogene"},{"key":"2021112215491924300_B2","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1038\/sj.onc.1202675","article-title":"The EWS\/TEC fusion protein encoded by the t(9;22) chromosomal translocation in human chondrosarcomas is a highly potent transcriptional activator","volume":"18","author":"Labelle","year":"1999","journal-title":"Oncogene"},{"key":"2021112215491924300_B3","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1093\/hmg\/4.12.2219","article-title":"Oncogenic conversion of a novel orphan nuclear receptor by chromosome translocation","volume":"4","author":"Labelle","year":"1995","journal-title":"Hum. 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