{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:14:28Z","timestamp":1791166468391,"version":"4.1.0"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:p>PRDM proteins have emerged as important regulators of disease and developmental processes. To gain insight into the mechanistic actions of the PRDM family, we have performed comprehensive characterization of a prototype member protein, the histone methyltransferase PRDM9, using biochemical, biophysical and chemical biology techniques. In the present paper we report the first known molecular characterization of a PRDM9-methylated recombinant histone octamer and the identification of new histone substrates for the enzyme. A single C321P mutant of the PR\/SET domain was demonstrated to significantly weaken PRDM9 activity. Additionally, we have optimized a robust biochemical assay amenable to high-throughput screening to facilitate the generation of small-molecule chemical probes for this protein family. The present study has provided valuable insight into the enzymology of an intrinsically active PRDM protein.<\/jats:p>","DOI":"10.1042\/bj20140374","type":"journal-article","created":{"date-parts":[[2014,5,6]],"date-time":"2014-05-06T09:26:16Z","timestamp":1399368376000},"page":"323-334","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Characterization of the histone methyltransferase PRDM9 using biochemical, biophysical and chemical biology techniques"],"prefix":"10.1042","volume":"461","author":[{"given":"Xiaoying","family":"Koh-Stenta","sequence":"first","affiliation":[{"name":"Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), Singapore 138669, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joma","family":"Joy","sequence":"additional","affiliation":[{"name":"Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 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