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Neutral amino acids, proline analogues, eflornithine and acivicin induced inward currents. For proline, glycine and tryptophan the apparent affinities and maximal transport rates increased with more negative membrane potentials. Proline-induced currents were dependent on pH, but not on sodium. Although proline represents the primary energy source of T. brucei in the tsetse fly, down-regulation of TbAAT6-expression by RNAi showed that in culture TbAAT6 is not essential for growth of procyclic form trypanosomes in the presence of glucose or proline as energy source. TbAAT6-RNAi lines of both bloodstream and procyclic form trypanosomes showed reduced susceptibility to eflornithine, whereas the sensitivity to acivicin remained unchanged, indicating that acivicin enters the cell by more than one transporter.<\/jats:p>","DOI":"10.1042\/bj20140719","type":"journal-article","created":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T08:50:16Z","timestamp":1404377416000},"page":"9-18","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["<i>Trypanosoma brucei<\/i> eflornithine transporter AAT6 is a low-affinity low-selective transporter for neutral amino acids"],"prefix":"10.1042","volume":"463","author":[{"given":"Christoph","family":"Mathieu","sequence":"first","affiliation":[{"name":"Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaia\u00a0Gonz\u00e1lez","family":"Salgado","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine, University of Bern, B\u00fchlstrasse 28, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corina","family":"Wirdnam","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Meier","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne\u00a0Suter","family":"Grotemeyer","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehud","family":"Inbar","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"M\u00e4ser","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstrasse 57, 4051 Basel, Switzerland"},{"name":"University of Basel, 4000 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zilberstein","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Sigel","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine, University of Bern, B\u00fchlstrasse 28, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"B\u00fctikofer","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine, University of Bern, B\u00fchlstrasse 28, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Rentsch","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2014,9,8]]},"reference":[{"key":"2021112216081032700_B1","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","article-title":"Human African trypanosomiasis","volume":"375","author":"Brun","year":"2010","journal-title":"Lancet"},{"key":"2021112216081032700_B2","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1038\/nbt0905-1089","article-title":"Protozoan genomics for drug discovery","volume":"23","author":"Chaudhary","year":"2005","journal-title":"Nat. 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