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The activation of ERK1\/2 is abolished by the GPR30 (G-protein-coupled receptor 30) antagonist G15 and GPR30 siRNA. When ER\u03b1 is down-regulated by prolonged treatment with E2 (oestradiol) or specific ER\u03b1 siRNA, the peptide response is blunted. Thus the simultaneous presence of GPR30 and ER\u03b1 is required for the action of ER\u03b117p. In addition, its PLM sequence, which interferes with the formation of the ER\u03b1\u2013calmodulin complex, appears to be requisite for the phosphorylation of ERK1\/2 and cell proliferation. Hence ER\u03b117p is, to our knowledge, the first known peptide targeting ER\u03b1\u2013GPR30 membrane cross-talk and the subsequent receptor-mediated biological effects.<\/jats:p>","DOI":"10.1042\/bj20150744","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T01:18:51Z","timestamp":1442279931000},"page":"97-109","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The sequence Pro295\u2013Thr311 of the hinge region of oestrogen receptor \u03b1 is involved in ERK1\/2 activation via GPR30\u00a0in leiomyoma cells"],"prefix":"10.1042","volume":"472","author":[{"given":"Denis","family":"Leiber","sequence":"first","affiliation":[{"name":"Laboratoire Signalisation et R\u00e9gulations Cellulaires, Institut de Biochimie et de Biologie Mol\u00e9culaire et Cellulaire, CNRS UMR 8619, Universit\u00e9 Paris-Sud, B\u00e2timent 430, 91405 Orsay Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Burlina","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s, UPMC Universit\u00e9 Paris 06, \u00c9cole Normale Sup\u00e9rieure, PSL Research University, CNRS UMR 7203, Laboratoire des Biomol\u00e9cules, Tour 23-33, 4 place Jussieu, 75252 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cillian","family":"Byrne","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s, UPMC Universit\u00e9 Paris 06, \u00c9cole Normale Sup\u00e9rieure, PSL Research University, CNRS UMR 7203, Laboratoire des Biomol\u00e9cules, Tour 23-33, 4 place Jussieu, 75252 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Robin","sequence":"additional","affiliation":[{"name":"Laboratoire Signalisation et R\u00e9gulations Cellulaires, Institut de Biochimie et de Biologie Mol\u00e9culaire et Cellulaire, CNRS UMR 8619, Universit\u00e9 Paris-Sud, B\u00e2timent 430, 91405 Orsay Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Piesse","sequence":"additional","affiliation":[{"name":"Institut de Biologie Int\u00e9grative (IFR 83), Sorbonne Universit\u00e9s - UPMC Univ Paris 06, IFR83, Case Courrier 25, 7 quai Saint-Bernard, 75252 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s, UPMC Universit\u00e9 Paris 06, \u00c9cole Normale Sup\u00e9rieure, PSL Research University, CNRS UMR 7203, Laboratoire des Biomol\u00e9cules, Tour 23-33, 4 place Jussieu, 75252 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Laboratoire de Canc\u00e9rologie Mammaire, Institut Jules Bordet, 1 rue H\u00e9ger Bordet, Brussels 1000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Tanfin","sequence":"additional","affiliation":[{"name":"Laboratoire Signalisation et R\u00e9gulations Cellulaires, Institut de Biochimie et de Biologie Mol\u00e9culaire et Cellulaire, CNRS UMR 8619, Universit\u00e9 Paris-Sud, B\u00e2timent 430, 91405 Orsay Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Jacquot","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s, UPMC Universit\u00e9 Paris 06, \u00c9cole Normale Sup\u00e9rieure, PSL Research University, CNRS UMR 7203, Laboratoire des Biomol\u00e9cules, Tour 23-33, 4 place Jussieu, 75252 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2015,10,30]]},"reference":[{"key":"2021112216254502000_B1","first-page":"119","article-title":"Tripartite steroid hormone receptor pharmacology: interaction with multiple effector sites as a basis for the cell- and promoter-specific action of these hormones","volume":"10","author":"Katzenellenbogen","year":"1996","journal-title":"Mol. 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