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In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin\u2013Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased both mZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells. Our results suggest that ZIP4 targeting may represent a new strategy to improve zinc absorption in humans.<\/jats:p>","DOI":"10.1042\/bj20150862","type":"journal-article","created":{"date-parts":[[2015,9,19]],"date-time":"2015-09-19T01:24:46Z","timestamp":1442625886000},"page":"183-193","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting"],"prefix":"10.1042","volume":"472","author":[{"given":"Ayako","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuma","family":"Ohkura","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Fukui Prefectural University, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Fukui Prefectural University, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumiko","family":"Kizu","sequence":"additional","affiliation":[{"name":"Department of Life and Living, Osaka Seikei College, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Narita","sequence":"additional","affiliation":[{"name":"Department of Food Science, Kyoto Women's University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichi","family":"Enomoto","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan"},{"name":"Center for Molecular Imaging Science, RIKEN Kobe Institute, Kobe, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusaku","family":"Miyamae","sequence":"additional","affiliation":[{"name":"Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Masuda","sequence":"additional","affiliation":[{"name":"Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Nagao","sequence":"additional","affiliation":[{"name":"Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Irie","sequence":"additional","affiliation":[{"name":"Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Ohigashi","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Fukui Prefectural University, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glen\u00a0K.","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiho","family":"Kambe","sequence":"additional","affiliation":[{"name":"Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2015,11,13]]},"reference":[{"key":"2021112216245831000_B1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1152\/physrev.1993.73.1.79","article-title":"The biochemical basis of zinc physiology","volume":"73","author":"Vallee","year":"1993","journal-title":"Physiol. 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