{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:06:33Z","timestamp":1770998793214,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,4,1]]},"abstract":"<jats:p>Hypolipidaemic drugs induce peroxisomal proliferation in the liver and many induce the formation of the hepatic endoplasmic reticulum in general and the formation of cytochrome P-450 in particular. We have induced the formation of rat liver microsomal cytochrome P-450 by the administration of the hypolipidaemic drug clofibrate, isolated the endoplasmic reticulum, solubilized the cytochrome P-450 from these membranes and subdivided the cytochrome P-450 into four fractions by the use of hydrophobic, anionic, cationic and adsorption chromatography. One of these fractions (cytochrome P-450 fraction 1) was highly purified to a specific content of 17nmol of cytochrome P-450\/mg of protein and the protein was active in a reconstituted enzyme system towards the 12- and 11-hydroxylation of the fatty acid, dodecanoic (lauric) acid, with preferential activity towards the 12-hydroxy metabolite. This reconstituted activity was absolutely dependent on NADPH, NADPH-cytochrome P-450 reductase and cytochrome P-450, indicating the role of the mixed-function oxidase system in the metabolism of lauric acid. Another fraction of the haemoprotein (cytochrome P-450 fraction 2) preferentially formed 11-hydroxylauric acid, whereas a third fraction (cytochrome P-450 fraction 3) exhibited only trace laurate oxidase activity and was similar to the phenobarbitone form of the haemoprotein in that these last two cytochromes rapidly turned-over the drug benzphetamine. The molecular weights and spectral properties of these cytochrome P-450 fractions are reported, along with the phenobarbitone-induced form of the enzyme and the nature of the cytochrome(s) induced by clofibrate pretreatment are discussed in the terms of possible haemoprotein heterogeneity.<\/jats:p>","DOI":"10.1042\/bj2030161","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:33:12Z","timestamp":1439238792000},"page":"161-168","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":192,"title":["Cytochrome <i>P<\/i>-450 induction by clofibrate. Purification and properties of a hepatic cytochrome <i>P<\/i>-450 relatively specific for the 12- and 11-hydroxylation of dodecanoic acid (lauric acid)"],"prefix":"10.1042","volume":"203","author":[{"given":"G. Gordon","family":"Gibson","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Division of Pharmacology and Toxicology, University of Surrey, Guildford GU2 5XH, Surrey, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry C.","family":"Orton","sequence":"additional","affiliation":[{"name":"ICI Pharmaceuticals Division, Department of Safety of Medicines, Alderley Park, Macclesfield, Cheshire, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul P.","family":"Tamburini","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Division of Pharmacology and Toxicology, University of Surrey, Guildford GU2 5XH, Surrey, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/203\/1\/161\/626197\/bj2030161.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/203\/1\/161\/626197\/bj2030161.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T12:54:08Z","timestamp":1637844848000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/203\/1\/161\/14165\/Cytochrome-P-450-induction-by-clofibrate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2030161","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1982,4,1]]}}}