{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:42:31Z","timestamp":1789504951309,"version":"build-2803163510"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,6,15]]},"abstract":"<jats:p>Adenosine (1.0\u2013100 \u03bcm). N6-phenylisopropyladenosine (0.1\u201310 \u03bcm) and 2-deoxyadenosine (10 mm) all produced a dose-dependent inhibition of glucose-stimulated insulin release. The inhibition of glucose-stimulated insulin release by adenosine and N6-phenylisopropyladenosine was abolished by 3-isobutyl-1-methylxanthine (0.1 mm), whereas 2-deoxyadenosine inhibited insulin release even in the presence of 3-isobutyl-1-methylxanthine. These adenosine nucleosides also inhibited the release of insulin induced by 4-methyl-2-oxopentanoate (20 mm), dl-glyceraldehyde (30 mm) and l-leucine (20 mm). Adenosine (10 \u03bcm). N6-phenylisopropyladenosine (10 \u03bcm) and 2-deoxyadenosine (10 mm) did not inhibit insulin biosynthesis or [U-14C]glucose oxidation at concentrations of the nucleosides that gave maximal inhibition of insulin release. However, adenosine, 2-deoxyadenosine and N6-phenylisopropyladenosine produced marked inhibition of the glucose-stimulated increases seen in islet cyclic AMP accumulation. Similar to its effects on insulin release, 3-isobutyl-1-methylxanthine (0.1 mm) antagonized the inhibitory effects of cyclic AMP accumulation produced by adenosine and N6-phenylisopropyladenosine, but had no effect on the inhibition of cyclic AMP accumulation seen with 2-deoxyadenosine. These results show that adenosine and its specifically modified analogues, 2-deoxyadenosine and N6-phenylisopropyladenosine, are strong inhibitors of insulin release from rat islets, a function that appears to be the consequence of their ability to inhibit the accumulation of cyclic AMP. It is proposed that the B cells, in common with many other tissues, may possess two different sites at which adenosine nucleosides interact to produce their biological effects; these are the so-called \u2018P\u2019 and \u2018R\u2019 sites first described by Londos &amp; Wolff [(1977) Proc. Natl. Acad. Sci. U.S.A.74, 5482\u20135486].<\/jats:p>","DOI":"10.1042\/bj2040689","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:33:24Z","timestamp":1439238804000},"page":"689-696","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Effects of adenosine, 2-deoxyadenosine and <i>N<\/i>6-phenylisopropyladenosine on rat islet function and metabolism"],"prefix":"10.1042","volume":"204","author":[{"given":"Iain L.","family":"Campbell","sequence":"first","affiliation":[{"name":"Department of Biochemistry, London Hospital Medical College, Turner Street, London E1 2AD, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, London Hospital Medical College, Turner Street, London E1 2AD, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/204\/3\/689\/577429\/bj2040689.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/204\/3\/689\/577429\/bj2040689.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T12:49:11Z","timestamp":1637844551000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/204\/3\/689\/14071\/Effects-of-adenosine-2-deoxyadenosine-and-N6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2040689","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1982,6,15]]}}}