{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T21:56:55Z","timestamp":1697925415458},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,11,15]]},"abstract":"<jats:p>Chicken tracheal mucosa in vitro transported and incorporated radioactive precursors into mucins, which were secreted at a steady rate into the tracheal lumen. Secretion of mucins labelled with 35S and 3H after pulse-labelling of the mucosal layer with Na235SO4 and d-[1-3H]glucosamine as precursors was an energy-dependent process, as it was strongly inhibited by the action of respiratory-chain inhibitors, an uncoupler of oxidative phosphorylation, a metabolic blocker and a temperature shift from 41\u00b0C to 5\u00b0C. On the other hand, both cholinergic and parasympathomimetic agents considerably increased the secretion of dual-radiolabelled mucins when applied on the submucosal side of the trachea. The effect of Ca2+ was directional, since only high submucosal (3.6 or 18mm) or low luminal (zero or 0.18mm) Ca2+ massively enhanced the secretion of radiolabelled mucin compared with the mucin output measured under physiological Ca2+ conditions (1.8mm). Whereas application of ionophore A23187 on either side of the trachea significantly increased mucin output, its presence in the appropriate tracheal compartment and under appropriate Ca2+ conditions further accentuated the output of radiolabelled mucins. Addition of acetylcholine under appropriate conditions also had an additive effect on the Ca2+-stimulated secretion of mucins. Ca2+ stimulation of mucin secretion appears to be dependent on the metabolic integrity of the mucosal cells. Mucins secreted in response to high submucosal and low luminal [Ca2+] appear to consist of a number of different types of glycoproteins, as judged from their ion-exchange-chromatographic behaviour.<\/jats:p>","DOI":"10.1042\/bj2080425","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:34:54Z","timestamp":1439238894000},"page":"425-433","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Directional Ca2+ effect on stimulation of mucin secretion from chicken trachea <i>in vitro<\/i>"],"prefix":"10.1042","volume":"208","author":[{"given":"Nasi","family":"Mian","sequence":"first","affiliation":[{"name":"Glycoprotein Research Unit, Science Laboratories, University of Durham, Durham DH1 3LE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Glycoprotein Research Unit, Science Laboratories, University of Durham, Durham DH1 3LE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Pope","sequence":"additional","affiliation":[{"name":"Glycoprotein Research Unit, Science Laboratories, University of Durham, Durham DH1 3LE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Glycoprotein Research Unit, Science Laboratories, University of Durham, Durham DH1 3LE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul S.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Physiology, St. George's Hospital Medical School, Tooting, London SW17 0QT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Balfre","sequence":"additional","affiliation":[{"name":"Department of Physiology, St. George's Hospital Medical School, Tooting, London SW17 0QT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul W.","family":"Kent","sequence":"additional","affiliation":[{"name":"Glycoprotein Research Unit, Science Laboratories, University of Durham, Durham DH1 3LE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/208\/2\/425\/784616\/bj2080425.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/208\/2\/425\/784616\/bj2080425.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T13:00:41Z","timestamp":1637845241000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/208\/2\/425\/14429\/Directional-Ca2-effect-on-stimulation-of-mucin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,11,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,11,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2080425","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1982,11,15]]}}}