{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T07:54:56Z","timestamp":1697961296532},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,10,1]]},"abstract":"<jats:p>We previously detected in cultured choriocarcinoma cells a 75000-Mr polypeptide defined by immunoblotting with antibody to a synthetic peptide Sp23 (Cys-Glu-Asn-Pro-Ser-Gln-Phe-Tyr-Glu-Asp-Leu) based on a cloned human endogenous proviral nucleotide sequence. On immunohistological staining, anti-Sp23 stains antigen(s) in the syncytiotrophoblasts of first-trimester placentas and in renal-cell adenocarcinoma tissues. The present report describes purification to homogeneity of the protein from cultured choriocarcinoma cells. The procedure involves extraction with non-ionic detergent and h.p.l.c. using, sequentially, gel-permeation, anion-exchange and reverse-phase columns. The yield was 110 micrograms\/g of total choriocarcinoma-cell protein. The results indicate that the purified protein is a monomeric and relatively hydrophilic molecule of Mr 75000.<\/jats:p>","DOI":"10.1042\/bj2310053","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:50:09Z","timestamp":1439239809000},"page":"53-57","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Purification, from cultured human choriocarcinoma cells, of a 75000-<i>M<\/i>r protein reacting with antibodies to a synthetic peptide based on a cloned human endogenous provirus nucleotide sequence"],"prefix":"10.1042","volume":"231","author":[{"given":"A","family":"N\u00e4rv\u00e4nen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/231\/1\/53\/585129\/bj2310053.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/231\/1\/53\/585129\/bj2310053.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T15:29:17Z","timestamp":1637854157000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/231\/1\/53\/20302\/Purification-from-cultured-human-choriocarcinoma"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2310053","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,10,1]]}}}