{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:30:34Z","timestamp":1760189434811},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1986,5,1]]},"abstract":"<jats:p>We have recently shown that the beta subunit of thyrotropin (TSH) can be cross-linked to the TSH receptor [Buckland, Strickland, Pierce &amp; Rees Smith (1985) Endocrinology (Baltimore) 116, 2122-2124; Buckland, Strickland &amp; Rees Smith (1985) Biochem. Soc. Trans. 13, 942-943]. We failed, however, to cross-link the alpha subunit to the receptor, leaving the role of this subunit in the TSH-TSH-receptor interaction uncertain. We now report the successful cross-linking of the TSH alpha subunit to the receptor by the use of two different cross-linking reagents. Our studies suggest therefore that both subunits of TSH form part of the hormone's receptor-binding site.<\/jats:p>","DOI":"10.1042\/bj2350879","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:53:25Z","timestamp":1439240005000},"page":"879-882","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Thyrotropin cross-links to the thyrotropin receptor through both the \u03b1 and \u03b2 subunits"],"prefix":"10.1042","volume":"235","author":[{"given":"P R","family":"Buckland","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C R","family":"Rickards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R D","family":"Howells","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B R","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/235\/3\/879\/586597\/bj2350879.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/235\/3\/879\/586597\/bj2350879.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T16:00:17Z","timestamp":1637856017000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/235\/3\/879\/21960\/Thyrotropin-cross-links-to-the-thyrotropin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,5,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2350879","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,5,1]]}}}