{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:25:35Z","timestamp":1760898335480},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,4,1]]},"abstract":"<jats:p>The presence of two essential tryptophan residues\/molecule was implicated in the binding site of Abrus agglutinin [Patanjali, Swamy, Anantharam, Khan &amp; Surolia (1984) Biochem. J. 217, 773-781]. A detailed study of the stopped-flow kinetics of the oxidation of tryptophan residues revealed three classes of tryptophan residues in the native protein. A discrete reorganization of tryptophan residues revealed three classes of tryptophan residues in the native protein. A discrete reorganization of tryptophan residues into two phases was observed upon ligand binding. The heterogeneity of tryptophan exposure was substantiated by quenching studies with acrylamide, succinimide and Cs+. Our study revealed the microenvironment of tryptophan residues to be hydrophobic, and also the presence of acidic amino acid residues in the vicinity of surface-localized tryptophan residues.<\/jats:p>","DOI":"10.1042\/bj2430079","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:58:17Z","timestamp":1439240297000},"page":"79-86","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Studies on tryptophan residues of <i>Abrus<\/i> agglutinin. Stopped-flow kinetics of modification and fluorescence-quenching studies"],"prefix":"10.1042","volume":"243","author":[{"given":"S R","family":"Patanjali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Swamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Surolia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/243\/1\/79\/589924\/bj2430079.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/243\/1\/79\/589924\/bj2430079.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:40:01Z","timestamp":1637862001000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/243\/1\/79\/23214\/Studies-on-tryptophan-residues-of-Abrus-agglutinin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2430079","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4,1]]}}}