{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:33:24Z","timestamp":1771644804709,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,8,1]]},"abstract":"<jats:p>Electron microscopy after rotary shadowing and negative staining of the large chondroitin sulphate proteoglycan from rat chondrosarcoma, bovine nasal cartilage and pig laryngeal cartilage demonstrated a unique multidomain structure for the protein core. A main characteristic is a pair of globular domains (diameter 6-8 nm), one of which forms the N-terminal hyaluronate-binding region. They are connected by a 25 nm-long rod-like domain of limited flexibility. This segment is continued by a 280 nm-long polypeptide strand containing most chondroitin sulphate chains (average length 40 nm) in a brush-like array and is terminated by a small C-terminal globular domain. The core protein showed a variable extent of degradation, including the loss of the C-terminal globular domain and sections of variable length of the chondroitin sulphate-bearing strand. The high abundance (30-50%) of the C-terminal domain in some extracted proteoglycan preparations indicated that this structure is present in the cartilage matrix rather than being a precursor-specific segment. It may contain the hepatolectin-like segment deduced from cDNA sequences corresponding to the 3\u2032-end of protein core mRNA [Doege, Fernandez, Hassell, Sasaki &amp; Yamada (1986) J. Biol. Chem. 261, 8108-8111; Sai, Tanaka, Kosher &amp; Tanzer (1986) Proc. Natl. Acad. Sci. 83, 5081-5085; Oldberg, Antonsson &amp; Heineg\u00e5rd (1987) Biochem. J. 243, 255-259].<\/jats:p>","DOI":"10.1042\/bj2450763","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:59:37Z","timestamp":1439240377000},"page":"763-772","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Extended and globular protein domains in cartilage proteoglycans"],"prefix":"10.1042","volume":"245","author":[{"given":"M","family":"Paulsson","sequence":"first","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"M\u00f6rgelin","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Wiedemann","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Beardmore-Gray","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Dunham","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hardingham","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Heineg\u00e5rd","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Timpl","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Engel","sequence":"additional","affiliation":[{"name":"Abteilung Biophysikalische Chemie, Biozentrum, Basel, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/245\/3\/763\/591557\/bj2450763.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/245\/3\/763\/591557\/bj2450763.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:56:49Z","timestamp":1637863009000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/245\/3\/763\/23555\/Extended-and-globular-protein-domains-in-cartilage"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2450763","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,8,1]]}}}