{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:55:51Z","timestamp":1792115751094,"version":"4.4.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,1,15]]},"abstract":"<jats:p>The purpose of these experiments was to determine whether the activity of the voltage-dependent Ca2+ channel was modulated in the same manner in islets of the ob\/ob mouse as in islets of homozygous lean mice of the same strain. The effect of agents that are known to alter the concentrations and movements of intracellular Ca2+ were investigated in relation to glucose-stimulated insulin secretion and in relation to the effect of forskolin. In islets of obese mice, verapamil and nifedipine both inhibited glucose-induced insulin release, nifedipine being the more potent inhibitor. Forskolin-stimulated secretion was inhibited either not at all (verapamil) or much less (nifedipine) in islets of the ob\/ob mouse compared with those of lean mice. At basal glucose concentrations, verapamil initiated insulin secretion in islets of the ob\/ob mouse and acted synergistically with forskolin to evoke a secretory activity that was 3-fold greater than that evoked by 20 mM-glucose. Nifedipine also initiated secretion at basal glucose concentrations and acted synergistically with forskolin, but its effect was considerably smaller than that of verapamil. A comparison of the effect of forskolin in the presence of Ca2+-channel blockers and in the absence of Ca2+ suggests that, in the obese mouse, the operation of the voltage-dependent Ca2+ channel is impaired.<\/jats:p>","DOI":"10.1042\/bj2490401","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:10:03Z","timestamp":1439241003000},"page":"401-407","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Different insulin-secretory responses to calcium-channel blockers in islets of lean and obese (<i>ob\/ob<\/i>) mice"],"prefix":"10.1042","volume":"249","author":[{"given":"M A","family":"Black","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Ottawa, Ottawa, K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L A","family":"Fournier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Ottawa, Ottawa, K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H M","family":"Heick","sequence":"additional","affiliation":[{"name":"the Eleanor M. Patterson Department of Laboratory Medicine, Children's Hospital of Eastern Ontario, Ottawa K1H 8L1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"B\u00e9gin-Heick","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Ottawa, Ottawa, K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/249\/2\/401\/592102\/bj2490401.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/249\/2\/401\/592102\/bj2490401.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:58:15Z","timestamp":1637863095000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/249\/2\/401\/23656\/Different-insulin-secretory-responses-to-calcium"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2490401","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,1,15]]}}}