{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T10:28:00Z","timestamp":1770978480564,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,7,15]]},"abstract":"<jats:p>We have studied synergism between adrenaline (epinephrine) and low concentrations of thrombin in gel-filtered human platelets prelabelled with [32P]Pi. Suspensions of platelets, which did not contain added fibrinogen, were incubated at 37 degrees C to measure changes in the levels of 32P-labelled phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP) and phosphatidate (PA), aggregation and dense-granule secretion after stimulation. Adrenaline alone (3.5-4.0 microM) did not cause a change in any parameter (phosphoinositide metabolism, aggregation and dense-granule secretion), but markedly enhanced the thrombin-induced responses over a narrow range of thrombin concentrations (0.03-0.08 units\/ml). The thrombin-induced hydrolysis of inositol phospholipids by phospholipase C, which was measured as the formation of [32P]PA, was potentiated by adrenaline, as was the increase in the levels of [32P]PIP2 and [32P]PIP. The presence of adrenaline caused a shift to the left for the thrombin-induced changes in the phosphoinositide metabolism, without affecting the maximal levels of 32P-labelled compounds obtained. A similar shift by adrenaline in the dose-response relationship was previously demonstrated for thrombin-induced aggregation and dense-granule secretion. Also, the narrow range of concentrations of thrombin over which adrenaline potentiates thrombin-induced platelet responses is the same for changes in phosphoinositide metabolism and physiological responses (aggregation and dense-granule secretion). Our observations clearly indicate that adrenaline directly or indirectly influences thrombin-induced changes in phosphoinositide metabolism.<\/jats:p>","DOI":"10.1042\/bj2530581","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:11:50Z","timestamp":1439241110000},"page":"581-586","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Synergism between thrombin and adrenaline (epinephrine) in human platelets. Marked potentiation of inositol phospholipid metabolism"],"prefix":"10.1042","volume":"253","author":[{"given":"V M","family":"Steen","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Bergen, Arstadveien 19, N-5009 Bergen, Norway."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O B","family":"Tysnes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bergen, Arstadveien 19, N-5009 Bergen, Norway."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Holmsen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bergen, Arstadveien 19, N-5009 Bergen, Norway."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/2\/581\/594019\/bj2530581.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/2\/581\/594019\/bj2530581.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:01:34Z","timestamp":1637863294000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/253\/2\/581\/23910\/Synergism-between-thrombin-and-adrenaline"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,7,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2530581","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7,15]]}}}