{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:47:23Z","timestamp":1760147243618},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,8,1]]},"abstract":"<jats:p>The interactions between Factor B (B), its activation products Ba and Bb, properdin (P) and C3i or C3b, components that together form the alternative-pathway C3 convertase enzyme of human complement, have been analysed. Fluid-phase complexes of the purified components C3i, B and P were probed with the homobifunctional cross-linking reagent disuccinimidyl tartarate, and efficient cross-linking of B to P was observed. The 140 kDa B-P conjugate formed was cleaved by Factor D to yield a single product of 85 kDa. This is consistent with a Ba-P heterodimer, and suggests that the initial interaction of B and P includes an interaction of P with the Ba domain of intact B. (The Ba fragment is not retained in the active P-stabilized complex, C3bBbP). By contrast, no cross-linking of P to the Bb domain of B could be demonstrated. Binding studies on cellular intermediates also provided evidence for a site of interaction between B and P, with high concentrations of B inhibiting P binding to EAC3b (sheep erythrocytes coated with antibody and C3b). Neither isolated Ba nor Bb had any effect on the P-EAC3b interaction. High concentrations of B also accelerated the decay of the functional EAC3bBbP complex. These data indicate that the positive co-operativity of binding to C3i or to C3b between B and P is mediated, at least in part, through a direct interaction between B and P.<\/jats:p>","DOI":"10.1042\/bj2530667","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:11:50Z","timestamp":1439241110000},"page":"667-675","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Analysis of the interaction between properdin and factor B, components of the alternative-pathway C3 convertase of complement"],"prefix":"10.1042","volume":"253","author":[{"given":"T C","family":"Farries","sequence":"first","affiliation":[{"name":"Mechanisms in Tumour Immunity Unit, MRC Centre, Hills Road, Cambridge CB2 2QH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Lachmann","sequence":"additional","affiliation":[{"name":"Mechanisms in Tumour Immunity Unit, MRC Centre, Hills Road, Cambridge CB2 2QH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R A","family":"Harrison","sequence":"additional","affiliation":[{"name":"Mechanisms in Tumour Immunity Unit, MRC Centre, Hills Road, Cambridge CB2 2QH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/3\/667\/593787\/bj2530667.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/3\/667\/593787\/bj2530667.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:54:49Z","timestamp":1637862889000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/253\/3\/667\/23481\/Analysis-of-the-interaction-between-properdin-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,8,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2530667","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,8,1]]}}}