{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:40:49Z","timestamp":1762674049033},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,8,1]]},"abstract":"<jats:p>The only known cellular action of AlF4- is to stimulate the G-proteins. The aim of the present work is to demonstrate that AlF4- also inhibits \u2018P\u2019-type cation-transport ATPases. NaF plus AlCl3 completely and reversibly inhibits the activity of the purified (Na+ + K+)-ATPase (Na+- and K+-activated ATPase) and of the purified plasmalemmal (Ca2+ + Mg2+)-ATPase (Ca2+-stimulated and Mg2+-dependent ATPase). It partially inhibits the activity of the sarcoplasmic-reticulum (Ca2+ + Mg2+)-ATPase, whereas it does not affect the mitochondrial H+-transporting ATPase. The inhibitory substances are neither F- nor Al3+ but rather fluoroaluminate complexes. Because AlF4- still inhibits the ATPase in the presence of guanosine 5\u2032-[beta-thio]diphosphate, and because guanosine 5\u2032-[beta gamma-imido]triphosphate does not inhibit the ATPase, it is unlikely that the inhibition could be due to the activation of an unknown G-protein. The time course of inhibition and the concentrations of NaF and AlCl3 required for this inhibition differ for the different ATPases. AlF4- inhibits the (Na+ + K+)-ATPase and the plasmalemmal (Ca2+ + Mg2+)-ATPase noncompetitively with respect to ATP and to their respective cationic substrates, Na+ and Ca2+. AlF4- probably binds to the phosphate-binding site of the ATPase, as the Ki for inhibition of the (Na+ + K+)-ATPase and of the plasmalemmal (Ca2+ + Mg2+)-ATPase is shifted in the presence of respectively 5 and 50 mM-Pi to higher concentrations of NaF. Moreover, AlF4- inhibits the K+-activated p-nitrophenylphosphatase of the (Na+ + K+)-ATPase competitively with respect to p-nitrophenyl phosphate. This AlF4- \u2013induced inhibition of \u2018P\u2019-type cation-transport ATPases warns us against explaining all the effects of AlF4- on intact cells by an activation of G-proteins.<\/jats:p>","DOI":"10.1042\/bj2530827","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:11:50Z","timestamp":1439241110000},"page":"827-833","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["AlF4- reversibly inhibits \u2018P\u2019-type cation-transport ATPases, possibly by interacting with the phosphate-binding site of the ATPase"],"prefix":"10.1042","volume":"253","author":[{"given":"L","family":"Missiaen","sequence":"first","affiliation":[{"name":"Department of Physiology"},{"name":"Department of Obstetrics and Gynecology, Catholic University of Leuven, B-3000 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Wuytack","sequence":"additional","affiliation":[{"name":"Department of Physiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"De Smedt","sequence":"additional","affiliation":[{"name":"Department of Physiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Vrolix","sequence":"additional","affiliation":[{"name":"Department of Physiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Casteels","sequence":"additional","affiliation":[{"name":"Department of Physiology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/3\/827\/593767\/bj2530827.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/253\/3\/827\/593767\/bj2530827.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:53:27Z","timestamp":1637862807000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/253\/3\/827\/23448\/AlF4-reversibly-inhibits-P-type-cation-transport"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,8,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2530827","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,8,1]]}}}