{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T14:27:23Z","timestamp":1649168843051},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,10,1]]},"abstract":"<jats:p>We studied the dephosphorylation of Ins(1,4,5)P3 (inositol 1,4,5-trisphosphate) by permeabilized rat intestinal epithelial cells incubated in a medium resembling intracellular ionic strength and pH. Saponin-permeabilized cells rapidly dephosphorylated Ins(1,4,5)P3 to a mixture of three InsP2 (inositol bisphosphate) isomers, namely Ins(1,4)P2, Ins(1,5)P2 and Ins(4,5)P2. These products were identified by h.p.l.c. analysis after dephosphorylation of both 3H- and 32P-labelled Ins(1,4,5)P3. Ins(1,4)P2 accumulated to about half of the concentration attained by Ins(1,5)P2 and Ins(4,5)P2. Ins(1,4,5)P3 dephosphorylation was inhibited, by up to 75%, by 10 mM-glucose 6-phosphate. In these conditions Ins(1,4)P2 became the predominant product, indicating that glucose 6-phosphate inhibited non-specific dephosphorylation of Ins(1,4,5)P3, at least at the 1- and 4-phosphate groups. Ins(1,4)P2 was further dephosphorylated, and the major InsP (inositol monophosphate) product was Ins4P. Most of the glucose 6-phosphate-inhibitable Ins(1,4,5)P3 phosphatase activity was exposed on the cell surface. The glucose 6-phosphate-insensitive Ins(1,4,5)P3 5-phosphatase activity was not detected until the cells were permeabilized with saponin. This intracellular 5-phosphatase activity was: (i) predominantly associated with the particulate portion of the cell; (ii) strongly inhibited by 10 mM-2,3-bisphosphoglycerate; (iii) insensitive to 50 mM-Li+. Therefore the Ins(1,4,5)P3 5-phosphatase activity in enterocytes appears similar to the 5-phosphatase that has been characterized in a number of cell types.<\/jats:p>","DOI":"10.1042\/bj2550131","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:12:33Z","timestamp":1439241153000},"page":"131-137","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["1<scp>d<\/scp>-<i>myo<\/i>-inositol 1,4,5-trisphosphate dephosphorylation by rat enterocytes involves an intracellular 5-phosphatase and non-specific phosphatase activity at the cell surface"],"prefix":"10.1042","volume":"255","author":[{"given":"C","family":"Rubiera","sequence":"first","affiliation":[{"name":"Departamento de Biologia Funcional, Universidad de Oviedo, 33071 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Velasco","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Funcional, Universidad de Oviedo, 33071 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R H","family":"Michell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Birmingham, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P S","family":"Lazo","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Funcional, Universidad de Oviedo, 33071 Oviedo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Shears","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Birmingham, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/1\/131\/593526\/bj2550131.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/1\/131\/593526\/bj2550131.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:57:15Z","timestamp":1637863035000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/255\/1\/131\/23589\/1d-myo-inositol-1-4-5-trisphosphate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2550131","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10,1]]}}}