{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:35:42Z","timestamp":1760985342894},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,11,1]]},"abstract":"<jats:p>Plasma-membrane fractions were prepared from the livers of rats injected with 0.15 M-NaCl (controls) or vasopressin (1 nmol\/kg body wt.). When assayed in the presence of deoxycholate, vasopressin increased the Vmax. of plasma-membrane diacylglycerol kinase 2-4-fold, and the apparent Km of the enzyme for 1,2-dioleoyl-sn-glycerol was doubled. The effect of vasopressin on the Vmax. of plasma-membrane diacylglycerol kinase was twice as great between pH 7 and 8.5 than at pH 6 or 6.5. Vasopressin doubled the activity of diacylglycerol kinase in the plasma-membrane fraction when the enzyme was assayed with phosphatidylserine rather than deoxycholate as stimulator, and when either 1-stearoyl-2-arachidonoyl-sn-glycerol or 1,2-dioleoyl-sn-glycerol was the substrate. In perfused livers vasopressin (10 nM) increased the Vmax. of plasma-membrane diacylglycerol kinase 2-fold, and phenylephrine (3 microM) gave a similar effect. Vasopressin doubled diacylglycerol kinase activity in hepatocytes that had been preincubated for 55 min, but not in cells that had only been preincubated for 15 min.<\/jats:p>","DOI":"10.1042\/bj2550923","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:12:33Z","timestamp":1439241153000},"page":"923-928","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Activation of rat liver plasma-membrane diacylglycerol kinase by vasopressin and phenylephrine"],"prefix":"10.1042","volume":"255","author":[{"given":"M H","family":"Rider","sequence":"first","affiliation":[{"name":"Hormone and Metabolic Research Unit, Institute of Cellular and Molecular Pathology (ICP) and Louvain University Medical School, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Baquet","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, Institute of Cellular and Molecular Pathology (ICP) and Louvain University Medical School, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/3\/923\/594341\/bj2550923.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/3\/923\/594341\/bj2550923.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:08:51Z","timestamp":1637863731000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/255\/3\/923\/24502\/Activation-of-rat-liver-plasma-membrane"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2550923","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11,1]]}}}