{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:59:50Z","timestamp":1760299190906},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,11,1]]},"abstract":"<jats:p>The glucose transporter has been identified in a variety of mammalian cell membranes using a photoactivatable carrier-free radioiodinated derivative of forskolin, 3-[125I]iodo-4-azidophenethylamido-7-O-succinyldeacetylforskoli n ([125I]IAPS-forskolin) at 1-3 nM. The membranes that were photolabelled with [125I]IAPS-forskolin were human placental membranes, rat cortical and cerebellar synaptic membranes, rat cardiac sarcolemmal membranes, rat adipocyte plasma membranes, smooth-muscle membranes, and S49 wild-type (WT) lymphoma-cell membranes. The glucose transporter in plasma membranes prepared from the insulin-responsive rat cardiac sarcolemmal cells, rat adipocytes and smooth-muscle cells were determined to be approx. 45 kDa by SDS\/polyacrylamide-gel electrophoresis (PAGE). Photolysis of human placental membranes, rat cortical and cerebellar synaptic membranes, and WT lymphoma membranes with [125I]-IAPS-forskolin, followed by SDS\/PAGE, indicated specific derivatization of a broad band (43-55 kDa) in placental membranes and a narrower band (approx. 45 kDa) in synaptic membranes and WT lymphoma membranes. Digestion of the [125I]IAPS-forskolin-labelled placental and WT lymphoma membranes with endo-beta-galactosidase showed a reduction in the apparent molecular mass of the radiolabelled band to approx. 40 kDa. The membranes that were photolabelled with [125I]IAPS-forskolin and trypsin-treated produced a radiolabelled proteolytic fragment with an apparent molecular mass of 18 kDa. [125I]IAPS-forskolin is a highly effective probe for identifying low levels of glucose transporters in mammalian tissues.<\/jats:p>","DOI":"10.1042\/bj2550983","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:12:33Z","timestamp":1439241153000},"page":"983-990","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of the glucose transporter in mammalian cell membranes with a125I-forskolin photoaffinity label"],"prefix":"10.1042","volume":"255","author":[{"given":"B E","family":"Wadzinski","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Wisconsin Medical School, Madison, WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M F","family":"Shanahan","sequence":"additional","affiliation":[{"name":"Department of Physiology, Southern Illinois University School of Medicine, Carbondale, IL 62901"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R B","family":"Clark","sequence":"additional","affiliation":[{"name":"Graduate School of Biomedical Sciences and the Pharmacology Department, University of Texas Health Center, Houston, TX 77025, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A E","family":"Ruoho","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Wisconsin Medical School, Madison, WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/3\/983\/594321\/bj2550983.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/255\/3\/983\/594321\/bj2550983.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:08:18Z","timestamp":1637863698000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/255\/3\/983\/24461\/Identification-of-the-glucose-transporter-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2550983","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11,1]]}}}