{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:05:29Z","timestamp":1758279929543},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,12,15]]},"abstract":"<jats:p>BBP-II, the major biotin-binding protein from chicken oocytes, was purified 12,000-fold with a 22% yield. The purification procedure includes butan-1-ol extraction of yolk lipids, phosphocellulose chromatography of the water-soluble proteins, DEAE-cellulose chromatography at pH 7.4 and hydroxyapatite column chromatography. Final purification was obtained by using a second DEAE-cellulose column chromatography at pH 6.0. BBP-I activity separated from BBP-II activity during elution from the first DEAE-cellulose column. Purified BBP-II was homogeneous on both polyacrylamide-gel electrophoresis and SDS\/polyacrylamide-gel electrophoresis under conditions that would detect a 1% impurity. The subunit Mr determined from SDS\/polyacrylamide-gel electrophoresis was 18,200 (72,600 for tetramer), which compares favourably with an Mr value of 17,300 (69,100) calculated from the amino acid analysis. A single precipitin line formed when rabbit antiserum to the protein was directed against a crude chicken egg-yolk sample. BBP-II purified by this procedure lacked carbohydrate and phosphate, was stable indefinitely when frozen, and was quite stable at room temperature. The N-terminal amino acid sequence showed polymorphism at three positions in the first 23 residues and was about 45% identical with the N-terminal 22 residues of avidin. Antiserum to BBP-II cross-reacted with BBP-I and similar proteins in the yolk of eggs from various birds and alligator as judged by immunodiffusion and enzyme-linked immunosorbent assays. No cross-reaction was observed with chicken egg-white by either of these methods.<\/jats:p>","DOI":"10.1042\/bj2560797","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:12:39Z","timestamp":1439241159000},"page":"797-805","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Purification and characterization of biotin-binding protein II from chicken oocytes"],"prefix":"10.1042","volume":"256","author":[{"given":"L","family":"Bush","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"McGahan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"3rd","given":"H B","family":"White","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/256\/3\/797\/596314\/bj2560797.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/256\/3\/797\/596314\/bj2560797.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:18:15Z","timestamp":1637864295000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/256\/3\/797\/25256\/Purification-and-characterization-of-biotin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,12,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,12,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2560797","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,12,15]]}}}