{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:57:33Z","timestamp":1791356253224,"version":"4.3.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,7,15]]},"abstract":"<jats:p>The e.p.r. signals attributable to a cytochrome bd-type ubiquinol: O2 oxidoreductase (cytochrome b-558-b-595-d) were studied in a cytoplasmic membrane preparation of Escherichia coli that had been grown on glycerol with fumarate as respiratory-chain oxidant. Two major high-spin ferric haem signals were resolved on the basis of their potentiometric behaviour: a rhombic high-spin species (gx = 6.25, gy = 5.54) was assigned to haem b-595, and an axial high-spin (gx = 5.97, gy = 5.96) species was assigned to the haem d. These signals titrated with Em.7 values of 154 and 261 mV respectively, corresponding closely to optically determined values for haem b-595 and haem d. At high potentials (greater than 300 mV) the rhombic species attributable to haem b-595 underwent a partial transition to a second rhombic species with g-values of 6.24 (gx) and 5.67 (gy). The high-spin ferric haem spectra were affected by O2, CO, cyanide and pH. A low-spin ferric haem signal was observed at g = 3.3 (gz), which titrated with an Em.7 of 226 mV, and this was assigned to haem b-558. The data support a model for cytochrome bd with two ligand-binding sites, a single haem d and a single haem b-595.<\/jats:p>","DOI":"10.1042\/bj2610437","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:13:56Z","timestamp":1439241236000},"page":"437-443","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The cytochromes of anaerobically grown <i>Escherichia coli<\/i>. An electron-paramagnetic-resonance study of the cytochrome <i>bd<\/i> complex <i>in situ<\/i>"],"prefix":"10.1042","volume":"261","author":[{"given":"R A","family":"Rothery","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of St. Andrews, St. Andrews, Fife KY16 9AL, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W J","family":"Ingledew","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of St. Andrews, St. Andrews, Fife KY16 9AL, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/261\/2\/437\/597232\/bj2610437.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/261\/2\/437\/597232\/bj2610437.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:17:24Z","timestamp":1637864244000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/261\/2\/437\/25193\/The-cytochromes-of-anaerobically-grown-Escherichia"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,7,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2610437","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7,15]]}}}