{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T18:03:29Z","timestamp":1772042609557,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,8,1]]},"abstract":"<jats:p>We have previously demonstrated that 3,5,3\u2032-tri-iodo-L-thyronine (T3) produces a very rapid and transient increase in calcium uptake and cytoplasmic free calcium concentration in the rat thymocyte, and have postulated that Ca2+-ATPase may contribute to the overall effect of T3 on cellular calcium metabolism. In the present study, we show that in the rat thymocyte, T3 increased plasma membrane Ca2+-ATPase activity. This effect of T3 was very rapid, seen at 30 s after the addition of the hormone, and was concentration-related, evident at a physiological concentration as low as 1 pM. Evaluation of the effect of several thyronine analogues on Ca2+-ATPase activity revealed the following order of potency: D-T3 greater than or equal to 3\u2032-isopropyl-L-T2 = L-T3 = L-T4 = D-T4 greater than L-rT3 greater than 3,5-L-T2 greater than DL-thyronine. Studies with the calmodulin antagonist trifluoperazine demonstrated that thymocyte Ca2+-ATPase activity and its stimulation by T3 are influenced by calmodulin. Other studies showed that several adrenergic agents, agonists and antagonists, had no effect on thymocyte Ca2+-ATPase activity and its stimulation by T3. From these and previous observations, we would suggest that in the rat thymocyte, the T3-induced increase in Ca2+-ATPase activity, which enhances the expulsion of calcium from the cell, plays a role in the diminution and transiency of the stimulatory effect of T3 on thymocyte calcium metabolism.<\/jats:p>","DOI":"10.1042\/bj2610749","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:13:56Z","timestamp":1439241236000},"page":"749-754","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Stimulation of calcium-ATPase activity by 3,5,3\u2032-tri-iodothyronine in rat thymocyte plasma membranes. A possible role in the modulation of cellular calcium concentration"],"prefix":"10.1042","volume":"261","author":[{"given":"J","family":"Segal","sequence":"first","affiliation":[{"name":"Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, The Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Hardiman","sequence":"additional","affiliation":[{"name":"Charles A. Dana Research Institute and the Harvard-Thorndike Laboratories of Beth Israel Hospital Department of Medicine, Beth Israel Hospital and Harvard Medical School, Boston, MA 02215, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Ingbar","sequence":"additional","affiliation":[{"name":"Charles A. Dana Research Institute and the Harvard-Thorndike Laboratories of Beth Israel Hospital Department of Medicine, Beth Israel Hospital and Harvard Medical School, Boston, MA 02215, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/261\/3\/749\/597414\/bj2610749.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/261\/3\/749\/597414\/bj2610749.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:21:20Z","timestamp":1637864480000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/261\/3\/749\/25517\/Stimulation-of-calcium-ATPase-activity-by-3-5-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,8,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2610749","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,8,1]]}}}