{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:46:50Z","timestamp":1760204810503},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,8,15]]},"abstract":"<jats:p>The capacity of three B-lymphocyte cell lines to generate superoxide (O2.-) was examined. The Burkitt lymphoma lines P.3HR-1 and Jijoye gave no response to phorbol 12-myristate 13-acetate (PMA) at 100 ng\/ml but produced up to 0.35 nmol of O2.-\/min per mg of protein when stimulated with 5 micrograms of PMA\/ml; the cell line RPMI 1788 produced Nitro Blue Tetrazolium-positive responses to low PMA concentrations and approx. 0.4 nmol of O2.-\/min per mg of protein at 5 micrograms of PMA\/ml. Each cell line contained approx. 10 pmol of low-potential cytochrome b (cytochrome b-245)\/mg of protein. Homogenates of PMA-activated cells gave 10-20-fold greater rates of O2.- produced per mg of protein. The Km for NADPH varied between approx. 250 microM for P3.HR-1 and RPMI 1788 cell lines and 30.5 +\/- 6.5 microM for the Jijoye cell line; the Km values for NADH were higher. Determination of intracellular NADPH concentration showed that this might limit the rate of O2.- production since in each cell line it was at or below the Km concentration.<\/jats:p>","DOI":"10.1042\/bj2620373","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:14:08Z","timestamp":1439241248000},"page":"373-375","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Properties of the superoxide-generating oxidase of B-lymphocyte cell lines. Determination of Michaelis parameters"],"prefix":"10.1042","volume":"262","author":[{"given":"J T","family":"Hancock","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F E","family":"Maly","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology, Inselspital Bern, CH-3010 Bern, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O T","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/262\/1\/373\/597731\/bj2620373.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/262\/1\/373\/597731\/bj2620373.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:35:26Z","timestamp":1637865326000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/262\/1\/373\/26249\/Properties-of-the-superoxide-generating-oxidase-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2620373","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,8,15]]}}}