{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:48:07Z","timestamp":1759682887058},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10,1]]},"abstract":"<jats:p>We have validated a quantitative \u2018in-situ\u2019 hybridization method and computer-assisted image analysis of autoradiographs as a technique for measuring atrial-natriuretic-factor (ANF) mRNA in tissue sections of rat heart by: (i) producing radioactive standards to calibrate the autoradiograms and (ii) assessing: (a) specificity (through RNAase A background subtraction, comparison of ANF mRNA and non-ANF mRNA probe binding to sections, Northern analysis and section-thickness titration curves); (b) sensitivity (by calculating the limit of detection for ventricular levels of ANF mRNA); (c) precision [inter-assay CV (coefficient of variation) less than 10%; intra-assay CV 6-7%]; and (d) accuracy. We have found with this technique that deoxycortone and saline treatment of rats elevates ANF mRNA to a larger extent in the ventricles than in the atria and that, in neonatal-rat hearts, ANF mRNA is elevated in all cardiac chambers relative to adult levels.<\/jats:p>","DOI":"10.1042\/bj2630121","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:14:21Z","timestamp":1439241261000},"page":"121-127","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["A quantitative <i>\u2018in-situ\u2019<\/i> hybridization method using computer-assisted image analysis. Validation and measurement of atrial-natriuretic-factor mRNA in the rat heart"],"prefix":"10.1042","volume":"263","author":[{"given":"D J","family":"Nunez","sequence":"first","affiliation":[{"name":"Clinical Pharmacology Unit, Addenbrooke's Hospital, Cambridge CB2 2QQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A P","family":"Davenport","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Unit, Addenbrooke's Hospital, Cambridge CB2 2QQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P C","family":"Emson","sequence":"additional","affiliation":[{"name":"MRC Group, Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Brown","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Unit, Addenbrooke's Hospital, Cambridge CB2 2QQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/1\/121\/598203\/bj2630121.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/1\/121\/598203\/bj2630121.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:08:21Z","timestamp":1637863701000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/263\/1\/121\/24466\/A-quantitative-in-situ-hybridization-method-using"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2630121","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,1]]}}}