{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:12:19Z","timestamp":1768399939585,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10,15]]},"abstract":"<jats:p>Liver endothelium can remove and transport the glycoprotein transferrin (TF). During this process the molecules are desialylated; however, in contrast with other such glycoproteins, for example caeruloplasmin, only half of transported TF is desialylated. To explore which component of TF is desialylated, we double-labelled fully sialylated TF with [3H]sialic acid residues and a 125I-protein moiety. This was then \u2018chased\u2019 through purified liver endothelium in pulse-chase experiments. Endothelium-conditioned TF was fractionated on an RCA120 affinity column into sialylated and desialylated components. Each component was then re-fractionated on a concanavalin A affinity column, which separates the glycoprotein according to the branching pattern of its glycan chain. The desialylated fraction was eluted only as a triantennary component, whereas the non-desialylated fraction consisted only of bi- and tetra-antennary chains. The significance of this selective desialylation of triantennary chain of TF in the subsequent metabolism of its iron content and its possible role in the pathogenesis of alcohol-induced hepatic siderosis are discussed.<\/jats:p>","DOI":"10.1042\/bj2630491","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:14:21Z","timestamp":1439241261000},"page":"491-496","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Desialylation of transferrin by liver endothelium is selective for its triantennary chain"],"prefix":"10.1042","volume":"263","author":[{"given":"S","family":"Irie","sequence":"first","affiliation":[{"name":"Veterans Administration Medical Center and University of Mississippi School of Medicine, Jackson, MS 39216, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Tavassoli","sequence":"additional","affiliation":[{"name":"Veterans Administration Medical Center and University of Mississippi School of Medicine, Jackson, MS 39216, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/2\/491\/598006\/bj2630491.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/2\/491\/598006\/bj2630491.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:10:22Z","timestamp":1637863822000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/263\/2\/491\/24643\/Desialylation-of-transferrin-by-liver-endothelium"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,10,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2630491","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,15]]}}}